Pathogenic variability of Colletotrichum lindemuthianum and Ecuadorian Phaseolus vulgaris L. germplasm resistance
DOI:
https://doi.org/10.15517/ma.v29i1.27511Keywords:
anthracnose, physiological races, genetic resistance, genetic variation, fungal diseases.Abstract
Anthracnose caused by Colletotrichum lindemuthianum is one of the most economically important diseases of bean (Phaseolus vulgaris L.) cultivation in Ecuador. The best control alternative is the use of resistant varieties. C. lindemuthianum presents great pathogenic variability, which hinders the development of varieties with a lasting resistance, therefore, the knowledge of the presence and distribution of the physiological races of the pathogen and the identification of resistance genes are key to developing varieties with broad and lasting resistance. The objective of this research was to determine the pathogenic variability of C. lindemuthianum and to evaluate the resistance of Ecuadorian bean germplasm. The research was carried out between 2013 and 2014. Seventeen isolates of C. lindemuthianum from northern central Ecuador were characterized by the inoculation of a group of twelve standard differential bean varieties. Among the analyzed samples, thirteen races were identified; five of those races had not been previously reported in the country. The differential G2333 (Co-42, Co-52 and Co-7) presented resistance to every characterized races in Ecuador. In addition, twenty - one improved varieties and elite bean lines were evaluated with sixteen of the seventeen isolates, three genotypes were identified (TB2, TB3 and INIAP 485 Urcuquí) with resistance to the mentioned isolates, which can be used as sources of resistance to Anthracnose. The identified sources of resistance in this study will allow to plan the development of bean varieties with broad and durable resistance to C. lindemuthianum.
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References
Araya, C.M. 2003. Coevolución de interacciones hospedante – patógeno en frijol común. Fitopatol. Bras. 28:221-228.
Awale, H., E. Falconí-Castillo, J. Villatoro-Mérida, y J. Kelly. 2008. Caracterización de aislamientos de Colletotrichum lindemuthianum de Ecuador y Guatemala para identificar genes de resistencia. Agron. Mesoam. 19:1-6. doi:https://doi.
org/10.15517/am.v19i1.5016
Balardin, R., A. Jarosz, and J.D. Kelly. 1997. Virulence and molecular diversity in Colletotrichum lindemuthianum from South,Central, and North America. Phytopathol. 87:1184-1191. doi:10.1094/PHYTO.1997.87.12.1184
Castaño, J. 1994. Principios básicos de fitopatología. 2da ed. Zamorano Academic Press, Zamorano, HON.
Campa, A., R. Giraldez, and J.J. Ferreira. 2011. Genetic analysis of the resistance to eight anthracnose races in the common bean differential cultivar Kaboon. Phytopathol. 101:757-764. doi:10.1094/PHYTO-11-10-0296
CIAT. 1987. Sistema estándar para la evaluación de germoplasma de frijol. CIAT, Cali, COL.
de-Freitas, MB, and M.J. Stadnik. 2012. Race-specific and ulvan-induced defense responses in bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum. Physiol. Mol. Plant Pathol. 78:8-13. doi:10.1016/j.pmpp.2011.12.004
Del-Río, L.E., R.S. Lamppa, and P.L. Gross. 2003. Characterization of the reaction of North Dakota dry bean cultivars to three races of Colletotrichum lindemuthianum. Plant Dis. 87:263-265. doi:10.1094/PDIS.2003.87.3.263
Falconi, E., J.B. Ochoa, E. Peralta, and D. Danial. 2003. Virulence patterns of Colletotrichum lindemuthianum in common bean in Ecuador. Annu. Rept. Bean Improv. Coop. 46:167-168.
Ferreira, J., A. Campa, and J. Kelly. 2013. Organization of genes conferring resistance to anthracnose in common bean. In: R.K. Varshney, and R. Tuberosa, editors, Translational genomic for crop breeding: biotic stress. John Wiley & Sons, Chichester, GBR. p. 151-182. doi:10.1002/9781118728475.ch9
Ferreira, J.J., A. Campa, E. Pérez-Vega, and R. Girales. 2008. Reaction of a bean germplasm collection against five races of Colletotrichum lindemuthianum identified in northern Spain and implications for breeding. Plant Dis. 92:705-708. doi:10.1094/PDIS-92-5-0705
Gallego, C., G.A. Ligarreto, L.N. Garzón, O.A. Oliveros, y L.J. Rincón. 2010. Rendimiento y reacción a Colletotrichum lindemuathianum en cultivares de fríjol voluble (Phaseolus vulgaris L.). Rev. Fac. Nal. Agron. Medellín 63:5477-5488.
Gonçalves-Vidigal, M.C., A.C. Meirelles, J.P. Poletine, L.L. De Sousa, A.S. Cruz, M.P. Nunes, G.F. Lacanallo, and P.S. Vidigal-Filho. 2012: Genetic analysis of anthracnose resistance in Pitanga dry bean cultivar. Plant Breed. 131:423-429. doi:10.1111/j.1439-0523.2011.01939.x
González, M., R. Rodríguez, F. Hernández, J. Acosta, O. Martínez, and J. Simpson 2004. Analysis of pathotypes of Colletotrichum lindemuthianum found in the Central Region of Mexico and resistance in elite germplasm of Phaseolus vulgaris. Plant Dis. 88:152-156. doi:10.1094/PDIS.2004.88.2.152
González, M., R. Rodríguez, M.E. Zavala, J.L. Jacobo, F. Hernández, J. Acosta, O. Martínez, and J. Simpson. 1998. Characterization of Mexican isolates of Colletotrichum lindemuthianum by using differential cultivars and molecular markers. Phytopathol. 88:292-299. doi:10.1094/PHYTO.1998.88.4.292
INIAP (Instituto Nacional de Investigaciones Agropecuarias). 2007. Informe anual de actividades 2006. INIAP, Programa Nacional de Leguminosas y Granos Andinos, Estación Experimental Santa Catalina, Quito, ECU.
INIAP (Instituto Nacional de Investigaciones Agropecuarias). 2010. Informe anual de actividades 2009. INIAP, Programa Nacional de Leguminosas y Granos Andinos, Estación Experimental Santa Catalina, Quito, ECU.
Kelly, J.D., L. Afanador, and L.S. Cameron. 1994. New races of Colletotrichum lindemuthianum in Michigan and implications in dry bean resistance breeding. Plant Dis. 78:892-894.
León, I. 2009. La antracnosis y la mancha angular del fríjol común (Phaseolus vulgaris L.). Temas Cienc. Tecnol. 13(39):45-54.
Mahuku, G., and J. Riascos. 2004. Virulence and molecular diversity within Colletotrichum lindemuthianum isolates from Andean and Mesoamerican bean varieties and regions. European J. Plant Pathol. 110:253-263. doi:10.1023/B:EJPP.0000019795.18984.74
Menezes, J., and J. Dianese. 1988. Race characterization of Brazilian isolates of Colletotrichum lindemuthianum and detection of resistance to anthracnose in Phaseolus vulgaris L. Phytopathol. 78:650-655.
Meneses, R., H. Waaijenberg, y L. Piérola. 1996. Las leguminosas en la agricultura boliviana: revisión de información. Impresores Colorgraf, Cochabamba, BOL.
Nayibe, L., M. Blair, y G. Ligarreto. 2007. Uso de selección asistida con marcadores para resistencia a antracnosis en fríjol común. Agron. Colomb. 25:207-214.
Pastor-Corrales, M.A. 1991. Estandarización de variedades diferenciales y designación de razas de Colletotrichum lindemuthianum. Phytopathol. 81:694.
Pastor-Corrales, M.A., O.A. Erazo, E.I. Estrada, and S.P. Singh. 1994. Inheritance of anthracnose resistance in common bean accession G 2333. Plant Dis. 78:959-962. doi:10.1094/PD-78-0959
Peralta, E., A. Murillo, E. Falconí, N. Mazón, y J. Pinzón. 2007. Manual de campo para el reconocimiento y control de las enfermedades más importantes que afectan al cultivo de fréjol (Phaseolus vulgaris L.) en Ecuador. Publicación Miscelánea No. 136. INIAP, Programa Nacional de Leguminosas y Granos Andinos, Estación Experimental Santa Catalina, Quito, ECU.
Pinto, J.M., R. Pereira, S.F. Mota, F.H. Ishikawa, and E.A. Souza. 2012. Investigating Phenotypic Variability in Colletotrichum lindemuthianum Populations. Phytopathol. 102:490-497.
Rodríguez-Guerra, R., J.A. Acosta-Gallegos, M.M. González-Chavira, y J. Simpson. 2006. Patotipos de Colletotrichum lindemuthianum y su implicación en la generación de cultivares resistentes de frijol. Agric. Téc. Méx. 32:101-114.
Sánchez-García, B.M., A. Flores-Olivas, A. Sánchez-Arizpe, S. Pineda-Rodríguez, G. López-Jiménez, S. Fraire-Velásquez, E.R. Garrido-Ramírez, J.A. Acosta-Gallegos, J. Simpson-Williamson, y R. Rodríguez-Guerra. 2009. Patotipos de Colletotrichum lindemuthianum en Oaxaca y San Luis Potosí, México, y resistencia en genotipos de frijol. Agric. Téc. Méx. 35:46-57.
Santana, G.E., y G. Mahuku. 2002. Diversidad de razas de Colletotrichum lindemuthianum en Antioquia y evaluación de
germoplasma de fréjol crema-rojo por resistencia a antracnosis. Agron. Mesoam. 13:95-103. doi:10.15517/am.v13i2.12024
Vega, L. 2011. Análisis de la resistencia de poblaciones locales de fréjol (Phaseolus vulgaris L.) de Cotacachi y Saraguro a antracnosis (Colletotrichum lindemuthianum) y roya (Uromyces appendiculatus). Tesis Ing. Agr. Universidad Central del Ecuador, Quito, ECU.
Young, R.A., M. Melotto, R.O. Nodari, and J.D. Kelly. 1998. Marker assisted dissection of oligogenic anthracnose resistance in the common bean cultivar G2333. Theor. Appl. Genet. 96:87-94. doi:10.1007/s001220050713
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