Bioformulated to maintain the viability of rhizobacteria and their application in Theobroma cacao L. CCN-51
DOI:
https://doi.org/10.15517/am.2024.56868Keywords:
microbiology, gene, pest control, biochemical substanceAbstract
Introduction. The use of bioformulated plant growth-promoting rhizobacteria is an alternative to reduce the dependence on pesticides in agriculture, due to their pathogen biocontrol and nutrient solubilizing action. Objective. To evaluate the effect of bioformulates on the cell viability of rhizobacteria and their effect on T. cacao L. Materials and methods. The research was developed out from January to December 2020 in the laboratories Microbiology and Molecular Biology of the State Technical University of Quevedo and in the farm "Ignolia", La Maná, Ecuador. The potential characteristics to be considered as a rhizobacteria were evaluated; identification of the chiA gene by Polymerase Chain Reaction; the effect of bioformulates on the cell viability of rhizobacteria and their application in the field to evaluate productivity and phytosanitary status of T. cacao. Results. Rhizobacteria had the capacity to solubilize of nutrients, producers of hydrolytic and biofilm-generating enzymes. Eighty percent of the strains presented the chiA gene, with antifungal activity against pathogenic fungi. The BIOQPGPRs bioformulated with A. calcoaceticus, E. asburiae, S. marcescens, P. protegens and P. veronii showed greater cell persistence (1.83E+5, 1.80E+5, 1.63E+5 and 1.63E+5) during the 26 days. Its edaphic application and injection in the rainy season increased leaf emissions with 100 and 108, and reduce the incidence of Phytophtora spp. and its edaphic application of the bacterial consortium improve dry grain yield (1270.6 kg/ha). Conclusions. BIOQPGPRs preserved rhizobacteria with cell viability for 26 days. Its field application increased the number of foliar emissions, the reduction of Phytophthora spp. incidence on pods and crop yield.
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Copyright (c) 2024 Hayron Fabricio Canchignia Martínez , Dayanara Nicolle Tapia Quintana , Javier Andrés Auhing Arcos , Cristhian John Macias Holguin , Ángel Virgilio Cedeño Moreira, Luis Fernando Vera Benites
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