Keywords
maintaining scorpions
captive scorpions
scorpion venom
venom extraction.
Escorpiones de Costa Rica
mantenimiento de escorpiones
cautiverio de escorpiones
veneno de escorpiones
extracción de veneno.
How to Cite
Abstract
Approximately 2 000 scorpion species can be found around the world; although few species are considered “harmful” to human beings, a high number of scorpionism cases are reported all over the world. The elaboration of anti-scorpion sera requires the establishment of an animal collection maintained in captivity for venom extraction purposes. The Clodomiro Picado Institute (ICP, for its acronym in Spanish), poses a vast trajectory in manufacturing snakebite antivenoms, and starts a scorpion collection in 2005 for this purpose. In total, 2 043 scorpions were classified in 11 species and collected during a seven-year period using a black-light flashlight and an intensive seeking methodology. The scorpions were collected from several localities of the Pacific and the Caribbean versants of Costa Rica. The venom extraction was performed by applying electrostimulation; the collected venom was characterized by total protein content in addition to median lethal doses. Centruroides bicolor showed higher amounts of venom yield, total protein content and more lethal dose, all of which were correlated with its body mass. The techniques used to keep scorpions in captivity allowed the animals to live several years. Longevity analysis showed significant differences among scorpion genera (H= 353.80; df= 3; P < 0.0001); moreover, the genus Didymocentrus lived longer with an average of 4.46 years. One key factor of its longevity was that it did not go through venom extraction processes. Additionally, a high survival rate of Tityus pachyurus born in captivity, compared to other species within the same genus, was observed (H= 94.32; df= 3; P < 0.0001). This characteristic should be taken into consideration, when programs of reproduction in captivity are designed. In conclusion, the maintenance of a scorpion collection was efficient for venom extraction purposes and a longer life expectancy of the animals. Moreover, there is a scarcity on publications regarding scorpion maintenance in captivity for venom extraction purposes; therefore, a deeper research in aspects such as reproduction, death causes and feeding behaviors is required.
References
Batista, C., Román-González, S., Salas-Castillo, S., Zamudio, F., Gómez-Lagunas, F., & Possani, L. (2007). Proteomic analysis of the venom from the scorpion Tityus stigmurus: Biochemical and physiological comparison with other Tityus species. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(1-2), 147-157.
Borges, A., Miranda, R., & Pascale, J. (2012). Scorpionism in Central America, with special reference to the case of Panama. Journal of Venomous Animals and Toxins including Tropical Diseases, 18(2), 130-143.
Candido, D., & Lucas, S. (2004). Maintenance of scorpions of the genus Tityus Koch (Scorpiones, Buthidae) for venom obtention at Instituto Butantan, Sao Paulo, Brazil. Journal of Venomous Animals and Toxins including Tropical Diseases, 10, 86-97.
Chippaux, J., & Goyffon, M. (2008). Epidemiology of scorpionism: A global appraisal. Acta Tropica, 107(2), 71-79.
Poggioli De Scannone, J. (1996). Suero antiescorpiónico. Desarrollo y producción. En Instituto Venezolano de Investigaciones Científicas (Ed.), Resúmenes del I Taller los Escorpiones y sus Toxinas. Biología, Clínica y Toxicología (p. 11). Caracas, Venezuela.
Dehghani, R., Vazirianzadeh, B., Nasrabadi, M., & Moravvej, S. (2010). Study of scorpionism in Kashan in central Iran. Pakistan Journal of Medical Sciences, 26(4), 955-958.
Fet, V., & Soleglad, M. (2005). Contributions to scorpion systematics: On recent changes in high-level taxonomy. Euscorpius, 31, 1-12. Retrieved from http://mds.marshall.edu/cgi/viewcontent.cgi?article=1025&context=euscorpius
Francke, O. F., & Stockwell, S. A. (1987). Scorpions (Arachnida) from Costa Rica. Special Publications the Museum Texas Tech University, 25, 1-64.
Francke, O. F. (1978). Systematic revision of diplocentrid scorpions (Diplocentridae) from Circum-Caribbean lands. USA: Texas Tech Press.14.
Gervais, P. (1843). Les principaux résultats d’un travail sur la famille des Scorpions. Comptes rendus hebdomadaires des séances de l'académie des sciences, 5(7), 129-131.
Gopalakrishnakone, P., Cheah, J., & Gwee, M. (1995). "Black scorpion (Heterometrus longimanus) as a laboratory animal: maintenance of a colony of scorpion for milking of venom for research, using a restraining device. Laboratory Animals, 29(4), 456-4588.
Isbister, G., Volschenk, E., & Seymour, J. (2004). Scorpion stings in Australia: five definite stings and a review. Internal Medicine Journal, 34(7), 427-430.
Kraepelin, K. (1911). Neue Beitrage zur systematik der gliederspinnen. Mitteilungen aus dem Naturhistorischen Museum in Hamburg, 28, 59-107.
Laïd, Y., Boutekdjiret, L., Oudjehane, R., Laraba-Djebari, F., Hellal, H., Guerinik, M., … Chippaux, J. (2012). Incidence and severity of scorpion stings in Algeria. Journal of Venomous Animals and Toxins including Tropical Diseases, 18(4), 399-410.
Leveridge, Y. (2000). Accidente y cuadro clínico por la picadura de alacranes en Costa Rica. Revista Costarricense de Ciencias Médicas, 21, 3-4.
Lourenço, R. W. (1982). Revision du genre Ananteris Thorell, 1891 (Scorpiones: Buthidae) et description de six especes nouvelles. Bulletin de Muséum National d’Histoire Naturelle, 4(1-2), 119-151.
Lourenço, R. W., Cloudsley-Thompson, J., Cuellar, O., Eickstedt, V., Barraviera, B., & Knox, M. (1996). The evolution of scorpionism in Brazil in recent years. Journal of Venomous Animals and Toxins including Tropical Diseases, 2(2), 121-134.
Lourenço, W. R. 1986. Tityus cerroazul, nouvelle espèce de Scorpion de Panama (Scorpiones, Buthidae). Bulletin de Muséum National d’Histoire Naturelle, 8(3), 637-641.
Oukkache, N., Chgoury, F., Lalaoui, M., Cano, A., & Ghalim, N. (2013). Comparison between two methods of scorpion venom milking in Morocco. Journal of Venomous Animals and Toxins including Tropical Diseases, 19(1), 1-5.
Pocock, R. (1898). Descriptions of some new scorpions from Central and South America. The Annals and magazine of natural history, 7(1), 384-394.
Polis, G. (1990). The Biology of Scorpions. California: Stanford University Press.
Rein, J. (2013). The Scorpion Files. Trondheim, Norwegian: University of Science and Technology. Retrieved from http://www.ntnu.no/ub/scorpion-files/
Rojas, G., Jiménez, J. M., & Gutiérrez, J. M. (1993). Caprylic acid fractionation of hyperimmune horse plasma: Description of a simple procedure for antivenom production. Toxicon, 32(3), 351-363.
Solano, G., Segura, A., Herrera, M., Gómez, A., Villalta, M., Gutiérrez, J. M., & León, G. (2010). Study of the design and analytical properties of the lethality neutralization assay used to estimate antivenom potency against Bothrops asper venom. Biologicals, 38, 577-585.
Víquez, C. (1999). Escorpiones de Costa Rica. Heredia, Costa Rica: INBIO.
Whittemore, F., Keegan, H., Fitzgerald, C., Bryant, H., & Flanigan, J. (1963). Studies of Scorpion Antivenins. Bulletin World Health Organization, 28, 505-511.
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