Conservación in vitro del cultivo de clavel español (Dianthus caryophyllus L.) a partir de sales minerales.

Liudmila Jiménez-Mariña; Juan José Silva-Pupo; Misterbino Borges-García; Milvia Fonseca-Arias

doi:10.15517/am.v27i1.21897

In vitro conservation of the cultivation of spanish carnation (Dianthus caryophyllus L.) from mineral salts.

Authors

Liudmila Jiménez-Mariña Instituto de Investigaciones Agropecuarias “Jorge Dimitrov”, Bayamo 85100, Granma, Cuba.
Juan José Silva-Pupo Universidad de Granma, Facultad de Ciencias Agrícolas, Centro de Estudios de Biotecnología Vegetal. Bayamo 85100 Granma, Cuba.
Misterbino Borges-García Universidad de Granma, Facultad de Ciencias Agrícolas, Centro de Estudios de Biotecnología Vegetal. Bayamo 85100 Granma, Cuba.
Milvia Fonseca-Arias Instituto de Investigaciones Agropecuarias “Jorge Dimitrov”, Bayamo 85100, Granma, Cuba.

DOI:

https://doi.org/10.15517/am.v27i1.21897

Keywords:

tissue culture, biological preservation, mineral nutrients.

Abstract

The objective of the present study was to determine the effect of the salts concentration described by Murashige and Skoog (MS) in short term in vitro conservation of nodal segments of Spanish Carnation (Dianthus caryophyllus L.). The investigation was developed in the Center of Vegetable Biotechnology Studies (CEBV), in the period 2012- 2013. The treatments consisted on the addition of MS salts (100; 75; 50; 25%) and a control (100% + growing regulators) in medium culture. Decreasing until 25% of salts concentration in culture medium reduced the growing speed, being able to conserve for six months under these conditions A survival and recovery of 91,3% was obtained.

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References

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Published

2015-12-09

How to Cite

Jiménez-Mariña, L., Silva-Pupo, J. J., Borges-García, M., & Fonseca-Arias, M. (2015). In vitro conservation of the cultivation of spanish carnation (Dianthus caryophyllus L.) from mineral salts. Agronomía Mesoamericana, 27(1), 177–181. https://doi.org/10.15517/am.v27i1.21897

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Issue

2016: Agronomía Mesoamericana: Vol. 27, Issue 1 (January-June)

Section

Short Communication

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