Lipid profile in milk of grazing cows in the colombian dry tropics


  • José Edwin Mojica-Rodríguez Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA)
  • Edwin Castro-Rincón Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA)
  • Juan Evangelista Carulla-Fornaguera Universidad Nacional de Colombia.
  • Carlos Eduardo Lascano-Aguilar Centro Internacional de Agricultura Tropical (CIAT).



fatty acids in bovine milk, animal feeding, forage, multiple purpose breeds


Introduction. The composition of fat in bovine milk has relevance in human health, where polyunsaturated fatty acids such as conjugated linoleic acid (ALC c9 t11) have positive effects (anticarcinogenic, antidiabetogenic). There are investigations regarding the effect of bovine feeding on milk quality in temperate zones (above 2200 m.a.s.l.), but few in the Colombian low tropics (below 1500 m.a.s.l.). Objetive. The objective of this work was to evaluate the effect of three grasses Mulato II, Tanzania, and Toledo on production, composition, and lipid panel in the milk of Gyr x Brown Swiss cows in the first-second-and three-third of lactation. Materials and methods. The study was conducted from May to June 2013 in the city council of San Diego, Cesar, Colombia. Two groups of nine animals were selected (three cows in each third lactation stage), and one cow was assigned to each grass in a replicated 3 x 3 Latin Square design. Results. Milk production was similar (p>0.05) in evaluated grasses and decreased (p<0.05) as the lactation advanced. The concentration of milk fat and protein was higher (p<0.05) in Tanzania and Mulato II, although in the second-third there was a higher content (p<0.05) of polyunsaturated acids, compared to Toledo. The lactation trimester did not affect (p>0.05) milk composition. There was higher fed (p<0.05) concentration of conjugated linoleic acid and polyunsaturated fatty acids in milk fat in cows fed with Tanzania and Toledo forage. Conclusion. Feeding dual-purpose cows with Tanzania and Toledo grasses could have positive effects on human health, due to the healthier lipid panel of the milk produced. 


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Allen, M.S. 2000. Effects of diet on short-term regulation of feed intake by lactating dairy cattle. J Dairy Sci. 83:1598-1624. doi:10.3168/jds.S0022-0302(00)75030-2

Amezquita, M. 1999. Diseño y análisis de ensayos para evaluación de pasturas en fincas. 7ma Reunión del Comité Asesor de la RIEPT. CIAT, COL (consultado 15 ene. 2018).

AOAC (Association Official of Analytical Chemistry). 2010. Official methods of analysis of AOAC International. 18th ed. AOAC Int., MD, USA.

Argel, J., W. Miles, D. Guiot, H. Cuadrado, y C. Lascano. 2007. Cultivar Mulato II (Brachiaria híbrido CIAT 36087): gramínea de alta calidad y producción forrajera, resistente a salivazo y adaptada a suelos tropicales ácidos bien drenados. CIAT, Cali, COL.

Bas, P., H. Archimede, A. Rouzeau, and D. Sauvant. 2003. Fatty acid composition of mixed-rumen bacteria: Effect of

concentration and type of forage. J. Dairy Sci. 86:2940-2948. doi:10.3168/jds.S0022-0302(03)73891-0

Benjamin, S., and F. Spener. 2009. Conjugated linoleic acids as functional food: an insight into their health benefits. Nutr. Metab. 6:36. doi:10.1186/1743-7075-6-36

Bessa, R.J.B., J. Silva, J.M.R. Ribeiro, and A.B. Portugal. 2000. Retículo rumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers. Livest. Prod. Sci. 63:201-211. doi:10.1016/S0301-


Bhattacharya, A., J. Banu, M. Rahman, J. Causey, and G. Fernandes. 2006. Biological effects of conjugated linoleic acids in helath and disease. J. Nutr. Biochem. 17:789-810. doi:10.1016/j.jnutbio.2006.02.009

Bilal, G., R. Cue, A. Mustafa, and J. Hayes. 2014. Effects of parity, age at calving and stage of lactation on fatty acid composition of milk Canadian Holsteins. Can. J. Anim. Sci. 94:401-406. doi:10.4141/cjas2013-172

Boufaied, H., P.Y. Chouinard, G.F. Tremblay, H.V. Petit, R. Michaud, and G. Belanger. 2003. Fatty acids in forages. I. Factors affecting concentrations. Can. J. Anim. Sci. 83:501-511. doi:10.4141/A02-098

Bunout, D., y E. Escobar. 2000. Prevención de enfermedades cardiovasculares: ¿Deben aplicarse los mismos criterios en América Latina que en Europa y Norte América? Rev. Esp. Cardiol. 53:889-895. doi:10.1016/S0300-8932(00)75171-2

Chilliard, Y., and A. Ferlay. 2004. Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reprod. Nutr. Dev. 44:467-492. doi:10.1051/rnd:2004052

Corl, B., L. Baumgard, D. Dwyer, J. Griinari. B. Phillips, and D. Bauman. 2001. The role delta 9 desaturase in the production of cis-9 trans-11 CLA. J. Nutr. Biochem. 12:622-630. doi:10.1016/S0955-2863(01)00180-2

CORPOICA (Corporación Colombiana de Investigación Agropecuaria). 2002. Atlas de los sistemas de producción bovina. Módulo Región Caribe. Plan de Modernización Tecnológica de la Ganadería Bovina. CORPOICA, Bogotá, COL.

CORPOICA (Corporación Colombiana de Investigación Agropecuaria). 2013. Informe anual proyectos de investigación en pastos y forrajes. CORPOICA, Bogotá, COL.

Díaz, G.G., R. Gutiérrez, N. Pérez, S. Vega, S. León, M. González, G. Prado, G. Urbán, A. Ramírez, y M. Pinto. 2002. Detección de adulteraciones en la grasa de leche pasteurizada mexicana. Rev. Salud Anim. 24(1):54-59.

Dilzer, A., and Y. Park. 2012. Implication of conjugated linoleic acid (CLA) in human health. Crit. Rev. Food Sci. Nutr. 52(6):488-513. doi:10.1080/10408398.2010.501409

Dohme, F., A. Machmuller, F. Sutter, and M. Kreuzer. 2004. Digestive and metabolic utilization of lauric, myristic and stearic acid in cows, and associated effects on milk fat quality. Arch. Anim. Nutr. 58(2):99-116. doi:10.1080/00039420410001667485

Doreau, M., and Y. Chilliard. 1997. Digestion and metabolism of dietary fat in farm animals. Br. J. Nutr. 78(1):15-35.


Elgersma, A., G. Ellen, H. Van Der Horst, H. Boer, P. Dekker, and S. Tamminga. 2004. Quick changes in milk fat composition from cows after transition from fress grass to a silage diet. Anim. Feed Sci. Technol. 117(1-2):13-27. doi:10.1016/j.anifeedsci.2004.08.003

Escobar, A., y J. Carulla. 2003. Efecto de la oferta de forraje sobre los parámetros productivos y composicionales de la leche en la sabana de Bogotá. Rev. Colom. Cienc. Pecu. 16:2721-2732. doi:10.21897/rmvz.273

Fawcet, B., and J.E. Scott. 1960. A rapid and precise method for determination of urea. J. Clin. Path. 13:156-159.

FEDEGAN (Federación de Ganaderos de Colombia). 2018. Consumo aparente per cápita anual de leche. Fondo estabilización de precios. FEDEGAN, COL. (consultado 15 ene. 2018).

Gagliostro, G., A. Rodríguez, P. Pellegrini, P. Gatti, G. Musset, R. Castañeda, A. Colombo, y Y. Chilliard. 2006. Efectos del suministro de aceite de pescado sólo o en combinación con aceite de girasol sobre las concentraciones de ácido linoleico conjugado (CLA) y omega 3 (n-3) en leche de cabra. Rev. Arg. Prod. Anim. 26(2):71-87.

Garcés, R., and M. Mancha. 1993. One step lipid extraction and fatty acid methyl esters preparation from fresh plant tissue. Anal. Biochem. 211:139-143. doi:10.1006/abio.1993.1244

Gastón, A.C., M.L. Pabón, and J.E. Carulla. 2014. Concentration of trans-vaccenic and rumenic acids in the milk from grazing cows supplemented with palm oil, rice bran or whole cottonseed. R. Bras. Zootec. 43(6):315-326. doi:10.1590/S1516-35982014000600006

Glasser, F., Ferlay A., Doreau M., Schmidely P., Sauvant D., and Chilliard Y. 2008. Long chain fatty acid metabolism in dairy cows: a meta-analisys of milk fatty acid yield in relation to duodenal flows and de novo synthesis. J. Dairy Sci. 91:2771-2785. doi:10.3168/jds.2007-0383.

Gómez, C., L. Bermejo, and V. Loria. 2011. Importance of a balance omega 6/omega 3 ratio for the maintenance of health: nutritional recommendations. Nutr. Hosp. 26:323-329. doi:10.1590/S0212-16112011000200013

Griinari, J.M., B.A. Corl, S.H. Lacy, P.Y. Chouinard, and K.V.V. Nurmela. 2000. Conjugated linoleic acid is synthesized

endogenously in lactating dairy cows by delta 9 desaturase. J. Nutr. 130:2285-2291. doi:10.1093/jn/130.9.2285

Haeiwa, H., T. Fujita, Y. Saitoh, and N. Miwa. 2014. Oleic acid promotes adaptability against oxidative stress in 3T3-L1 cells through lipohormesis. Mol. Cell. Biochem. 386:73-83. doi:10.1007/s11010-013-1846-9

Harvatine, K.J., Y.R. Boisclair, and D.E. Bauman. 2009. Recents advances in the regulation of mill fat synthesis. Animal 3:40-54. doi:10.1017/S1751731108003133

Hurley, W.L., G.J. Warner, and R.R. Grummer. 1987. Changes in triglyceride fatty acid composition of mammary secretions during involution. J. Dairy Sci. 70:2406-2410. doi:10.3168/jds.S0022-0302(87)80302-8

Huth, P., and K. Park. 2012. Influence of dairy product and milk fat consumption on cardiovascular disease risk: A review of the evidence. Adv. Nutr. 3:266-285. doi:10.3945/an.112.002030

Jeffery, H.J. 1970. The length of change-over periods in change-over designs with grazing cattle. Austr. J. Exp. Agric. Anim. Husb. 10:691-693. doi:10.1071/EA9700691

Jutzeler, R., and P. Colombani. 2010. Grass-based ruminant production methods and human bioconversion of vaccenic acid and estimations of maximal dietary intake of conjugated linoleic acids. Int. Dairy J. 20:433-448. doi:10.1016/j.idairyj.2010.01.008

Kay, J.K., W.J. Weber, C.E. Moore, D.E. Bauman, and L.B. Hansen. 2005. Effects of week of lactation and genetic selection for milk yield on milk fatty acid composition in Holstein cows. J. Dairy Sci. 83:3886-3893. doi:10.3168/jds.S0022-0302(05)73074-5

Kelly, M.L., J.R. Belly, D.A. Dwyer, J.M. Griinari, P.Y. Chouinard, M.E. Van-Amburgh, and D.E. Bauman. 2008. Dietary

fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J. Nutr. 128:881-885. doi:10.1093/jn/128.5.881

Kgwatalala, P.M., E.M. Ibeagua-Awemu, A.F. Mustafa, and X. Zhao. 2009. Influence of stearoil-coenzime A desaturase 1 genotipe and stage of lactation on fatty acid composition of Canadian Jersey cows. J. Dairy Sci. 92:1220-1228. doi:10.3168/jds.2008-1471

Khan, N.A., N.W. Farooq, M. ALI, M. Suleman, N. Ahmad, S.M. Sulaiman, N.W. Cone, and W.H. Hendriks. 2015. Effect of species and harvest maturity on the fatty acids profile of tropical forages. J. Anim. Plant Sci. 25:739-746.

Kratz, M., T. Baars, and S. Guyenet. 2013. The relationship between high-fat dairy consumption and obesity, cardiovascular, and metabolic disease. Eur. J. Nutr. 52:1-24. doi:10.1007/s00394-012-0418-1

León, C.J.M. 2011. Efecto de la incorporación de leguminosas en pasturas de trópico alto sobre el contenido de ácido linoleico conjugado ALC en la leche. Tesis MSc., Universidad Nacional de Colombia, Bogotá, COL.

Lock, A., C. Preseault, J. Rico, K. DeLand, and M. Allen. 2013. Feeding a C16:0-enriched fat supplement increased the yield of milk and improved conversion of feed to milk. J. Dairy Sci. 96:6650-6659. doi:10.3168/jds.2013-6892

López, E. 2010. Health effects of oleic acid and long chain omega-3 fatty acids /EPA and DHA) enriched milks. A review of intervention studies. Pharmacol. Res. 61:200-207. doi:10.1016/j.phrs.2009.10.007

Mahecha, L., J. Angulo, B. Salazar, M. Cerón, J. Gallo, C.H. Molina, E.J. Molina, J.H. Suárez, J.J. Lopera, and M. Olivera. 2008. Supplementation with bypass fat in silvopastoral systems diminishes the ratio of milk saturated/unsaturated fatty acids. Trop. Anim. Health Prod. 40:209-216. doi:10.1007/s11250-007-9082-5

Mendoza, C., M. Pabón, y F. Carulla. 2011. Variaciones diarias de la oferta forrajera, efecto sobre la producción y calidad de la leche. Rev. MVZ Córdoba 16:2721-2732. doi:10.21897/rmvz.273

Micha, R., and D. Mozzafrarian. 2010. Saturated fat and cardiometabolic risk factors, coronary heart desease, stroke and diabetes: a fresh look at the evidence. Lipids 45:893-905. doi:10.1007/s11745-010-3393-4

Mojica, J.E., E. Castro, J. Carulla, y C. Lascano. 2017. Efecto de la edad de rebrote sobre el perfil de ácidos grasos en gramíneas tropicales. Corpoica Cienc. Tecnol. Agropecu. 18:217-232. doi:10.21930/rcta.vol18_num2_art:623

Mojica, J.E., E. Castro, J. León, E. Cárdenas, M.L. Pabón, y J.E. Carulla. 2009. Efecto de la oferta de pasto kikuyo (Pennisetum clandestinum) sobre la producción y calidad composicional de la leche bovina. Livest. Res. Rural Dev. 21(1). (consultado 15 ene. 2018).

Mojica, R.J.E., R.E. Castro, Z.J. Silva, H.C. Hanzel, y Q.L. García. 2013. Producción y calidad composicional de la leche en ganaderías doble propósito del departamento del Cesar. CORPOICA, Cesar, COL. doi:10.21930/978-958-740-164-6

Mosley, E.E., B. Shaffi, P. Moate, and M. McGuire. 2006. Cis 9 Trans 11 Conjugated linoleic acid is synthesized directly from acid vaccenic acid in lactating dairy cattle. J. Nutr. 136:570-575. doi:10.1093/jn/136.3.570

Mosquera, P., y C. Lascano. 1992. Producción de leche de vacas en pasturas de Brachiaria decumbens solo y con acceso controlado a bancos de proteína. Rev. Past. Trop. 14(1):2-10.

Mozzafrarian, D., R. Micha, and S. Wallace. 2010. Effects of coronary heart disease of increasing polyunsaturated fat in place of saturated fat: A systematic review and meta-analysis of randomized controlled trials. PLoS Med. 7(3):e1000252. doi:10.1371/journal.pmed.1000252

Ntambi, J., and M. Makoto. 2003. Recent insights into stearoyl-CoA desaturase-1. Curr. Opin. Lipidol. 14:255-261.


Ørskov, E.R., F.D. Deb Howell, and F. Mould. 1980. The use of the nylon bag technique for the evaluation of feedstuffs. Trop. Anim. Prod. 5(3):295-213.

Ortega, R., J. Espinoza, E. Palacios, A. Palacios, O. Arjona, B. Murillo, y F. Rivera. 2013. Perfil de ácidos grasos en leche de vacas Chinampas (Bos taurus) alimentadas con forraje fresco de matorral sarcocaulescente o heno de alfalfa. Arch. Med. 45:45-51. doi:10.4067/S0301-732X2013000100008

Palmquist, D.L., A.D. Beaulieu, and D.M. Barbano. 1993. Feed and animal factors influencing milk fat composition. J. Dairy Sci. 76:1753-1771. doi:10.3168/jds.S0022-0302(93)77508-6

Parales, J. 2015. Efecto de la suplementación de aceites vegetales y sus mezclas sobre la fermentación ruminal y el perfil de ácidos grasos en leche. Tesis MSc., Universidad Nacional de Colombia, Bogotá, COL.

Prieto-Manrique, E., J.E. Vargas-Sánchez, J. Ángulo-Arizala, y L. Mahecha-Ledesma. 2017. Aceites vegetales sobre ácidos grasos y producción de metano in vitro en vacas lecheras. Agron. Mesoam. 28:1-18. doi:10.15517/am.v28i1.22034

Rego, O., A. Cabrita, H. Rosa, S. Anes, V. Duarte, A. Fonseca, C. Vouzela, F. Rocha, and R. Bessa. 2016. Changes in milk production and milk fatty acid composition of cows switched from pasture to a total mixed ration diet and back to pasture. Ital. J. Anim. Sci. 15:76-86. doi:10.1080/1828051X.2016.1141330

Rico, D.E. 2013. Recovery of normal ruminal biohydrogenation and the novo fatty acid synthesis following induction of milk fat depression in dairy cows. Ph.D. Diss., The Pennsylvania State University, PA, USA.

Rico, J.E., B. Moreno, M.L. Pabón, y J. Carulla. 2007. Composición de la grasa láctea de la sabana de Bogotá con énfasis en ácido ruménico - CLA cis-9, trans-11. Rev. Colomb. Cienc. Pecu. 20(1):30-39.

Roncallo, F.B., J. Barros, R. Bonilla, J. Murillo, y R. Del-Toro. 2009. Evaluación de arreglos silvopastoriles en explotaciones ganaderas de la microrregión bajo Magdalena. Corpoica Cienc. Tecnol. Agropecu. 10(1):60-69. doi:10.21930/rcta.vol10_num1_art:130

SAS. 2011. SAS/STAT, Software Versión 9.3. SAS Institute Inc., Cary, NC, USA.

Scerra, M., L. Chies, P. Caparra, C. Cilione, and F. Foti. 2016. Effect of only pasture on fatty acid composition of cow milk and Ciminà Caciocavallo Cheese. J. Food Res. 5(3):20-28. doi:10.5539/jfr.v5n3p20.

Shingfield, K.J., M. Bonnet, and N.D. Scollan. 2013. Recent developments in altering the fatty acid composition of ruminantderived foods. Animal. 7:132-162. doi:10.1017/S1751731112001681

Schmidely, P., F. Glasser, M. Doreau, and D. Sauvant. 2008. Digestion of fatty acids in ruminants: a meta-analysis of flows and variation factors. 1. Total fatty acids. Animal 2:677-690. doi:10.1017/S1751731108001717

Schroeder, G.F., G.A. Gagliostro, F. Bargo, J.E. Delahoy, and L.D. Muller. 2004. Effects of fat supplementation on milk

production and composition by dairy cows on pasture: a review. Livest. Prod. Sci. 86:1-18. doi:10.1016/S0301-6226(03)00118-0

Simopoulos, A. 2002. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed. Pharmacother. 56(8):365-379. doi:10.1016/S0753-3322(02)00253-6

Siri, P., Q. Sin, F. Hu, and R. Krauss. 2010. Meta-analysis of prospective cohort studies evaluating the association of saturated fat with cardiovascular disease. Am. J. Clin. Nutr. 91:535-546. doi:10.3945/ajcn.2009.27725

Smit, L., A. Baylin, and H. Campos. 2015. Conjugated linoleic acid in adipose tissue and risk of myocardial infarction. Am. J. Clin. Nutr. 92:34-40. doi:10.3945/ajcn.2010.29524

Soedamah-Muthu, S.S., E.L. Ding, W.K. Al-Delaimy, F.B. Hu, M.F. Engberink, W.C. Willett, and J.M. Geleijnse. 2011. Milk and dairy consumption and incidence of cardiovascular diseases and all-cause mortality: dose response meta-analysis of prospective cohort studies. Am. J. Clin. Nutr. 93:158-71. doi:10.3945/ajcn.2010.29866

Stoobs, T.H., and R.L. Sandland. 1972. The use of a latin square change - over design with dairy cows to detect differences in the quality of tropical pastures. Austr. J. Exp. Agric. Anim. Husb. 12:463-469. doi:10.1071/EA9720463

Stoop, W.M., H. Bovenhius, J.M.L. Heck, and J.A.M. Van-Arendock. 2009. Effect of lactation stage and energy status on milk fat composition of Holstein - Friesian cows. J. Dairy Sci. 92:1469-1478. doi:10.3168/jds.2008-1468

Toledo, J.M., and R. Schultze-Kraft. 1982. Metodología para la evaluación agronómica de pastos tropicales. CIAT, Cali, COL.

Ulbricht, T.L.V., and D.A.T. Southgate. 1991. Coronary heart disease: seven dietary factors. Lancet. 338:985-992. doi:10.1016/0140-6736(91)91846-M

Van-Soest, P.J., J. Roberton, and M. Lewis. 1991. Methods for dietary fiber, neutral fiber and no starch polysaccharides in relation to nutrition. J. Dairy Sci. 74:3583-3597. doi:10.3168/jds.S0022-0302(91)78551-2

Varádyová, Z., S. Kisidayova, P. Siroka, and D. Jalc. 2008. Comparison of fatty acid composition of bacterial and protozoal fractions in rumen fluid of sheep fed diet supplemented with sunflower, rapeseed and linseed oils. Anim. Feed Sci. Technol. 144:44-54. doi:10.1016/j.anifeedsci.2007.09.033

Yamasaki, M., K. Kishihara, I. Ikeda, M. Sugano, and K. Yamada. 1999. A recommended esterification method for gas

chromatographic measurement of conjugated linoleic acid. J. Am. Oil Chem. Soc. 76:933-938. doi:10.1007/s11746-999-0109-0



How to Cite

Mojica-Rodríguez, J. E., Castro-Rincón, E., Carulla-Fornaguera, J. E., & Lascano-Aguilar, C. E. (2019). Lipid profile in milk of grazing cows in the colombian dry tropics. Agronomía Mesoamericana, 30(2), 497–515.

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