Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colombia

Authors

  • Laura C. Mazo Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D.C., 110231, Colombia
  • Alberto Gómez Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D.C., 110231, Colombia. Iniciativa Genómica Javeriana, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D. C., 110231, Colombia.
  • Sonia R. Quintanilla Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D.C., 110231, Colombia. Iniciativa Genómica Javeriana, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D. C., 110231, Colombia.
  • Jaime E. Bernal Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D.C., 110231, Colombia. Iniciativa Genómica Javeriana, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D. C., 110231, Colombia.
  • Pedro Ortiz Valdivieso Iniciativa Genómica Javeriana, Pontificia Universidad Javeriana, Cra 7 # 40-62, edificio 32, Bogotá D. C., 110231, Colombia. Asociación Bogotana de Orquideología, Avenida calle 63 # 68G-14, Bogotá D. C., Colombia.

DOI:

https://doi.org/10.15517/lank.v0i0.11762

Keywords:

dna, adna, orchids, matk, exsiccates, herbarium

Abstract

Plant tissue from herbarium specimens contains DNA that has undergone post-mortem degradation. Only small amounts of possibly degraded genetic material free of chemicals and impurities can be extracted from these samples. The aim of the present work was to compare and determine which one of three previously published plant DNA extraction protocols would extract good quality DNA from orchid herbarium specimens stored up to 63 years, susceptible of PCR amplification and Sanger sequencing. The most effective protocol for this type of samples allowed us to obtain and sequence amplified products in 6 of the 7 samples studied with an optimal DNA / protein purity relationship.

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How to Cite

Mazo, L. C., Gómez, A., Quintanilla, S. R., Bernal, J. E., & Ortiz Valdivieso, P. (2013). Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colombia. Lankesteriana: International Journal on Orchidology, 12(2). https://doi.org/10.15517/lank.v0i0.11762

Issue

2012: Lankesteriana: Volumen 12, Número 2

Section

Articles

License

According to the Open Access policy promoted by the University of Costa Rica, all the papers published by Lankesteriana are licensed under the Creative Commons copyright and can be downloaded free of charge. The journal holds copyright and publishing rights under the CC BY-NC-ND 3.0 CR license.

Before the publication of the materials submitted by the author(s) in LANKESTERIANA, the author(s) hereby assign all rights in the article to the Lankester Botanical Garden.

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