A novel seed baiting technique for the epiphytic orchid <i>Rhynchostele cervantesii</i>, a means to acquire mycorrhizal fungi from protocorms
DOI:
https://doi.org/10.15517/lank.v15i1.18525Keywords:
Orchid, Epiphytic, Seed, Baiting, Mycorrhiza, RhynchosteleAbstract
We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.
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