Agronomic response of Phaseolus vulgaris L. to VIUSID® Agro application and chemical fertilization
DOI:
https://doi.org/10.15517/fy457807Keywords:
amino acids, foliar application, biostimulants, mineral fertilizer, common beanAbstract
Introduction. The use of biostimulants has emerged as a promising strategy to enhance common bean productivity. Objective. To evaluate the effect of different chemical fertilization levels combined with foliar application of VIUSID¨®Agro on the morpho-productive variables of bean, as well as analyze their relationships through a multivariate approach. Materials and methods. Between December 2021 and March 2022, in Sancti Spíritus, Cuba, an experiment was conducted using a randomized complete block design with a 3 × 2 factorial arrangement and five replications and five replications. Three levels of chemical fertilization were combined with two doses of VIUSID®Agro to evaluate the morpho-productive variables. Data were analyzed using two-way analysis of variance, Pearson correlation analysis, and principal component analysis (PCA). Results. The application of V₂, compared with V₁, increased plant height by 14, 11, and 7%, leaf area by 22, 15, and 11%, fresh biomass by 17, 15, and 18%, and dry biomass by 12, 13, and 18% under Fq₃, Fq₂, and Fq₁, respectively. V₁ outperformed V₂ in reproductive performance, increasing the number of pods by 32, 25, and 15%, seed mass by 13, 8, and 10%, and grain yield by 17, 15, and 18% under Fq₃, Fq₂, and Fq₁, respectively. Yield was positively correlated with seed mass and number of pods, while the first two principal components explained more than 70 % of the total variability, indicating an integrated response of the production system. Conclusions. The application of VIUSID¨®Agro decreased fertilization by 25 % and increased bean productivity.
Downloads
References
Abbas, M. S., Badawy, R. A., Abdel-Lattif, H. M., & El-Shabrawi, H. M. (2021). Synergistic effect of organic amendments and biostimulants on faba bean grown under sandy soil conditions. Scientia Agricola, 79(3), Artículo e20200300. https://doi.org/10.1590/1678-992X-2020-0300
Abreus Hernández, L., Calero Hurtado, A., Peña Calzada, K., Espinosa Negrín, A. M., & Jiménez Hernández, J. (2026). Combined fertilization with filter cake, microbial consortium, and amino acids improves peanut performance under water scarcity conditions. Stresses, 6(2), Artículo 19. https://doi.org/10.3390/stresses6020019
Acevedo González, Y., Pérez Díaz, Y., Calero Hurtado, A., & Peña Calzada, K. (2025). Densidades de plantas y fertilizantes en la producción sostenible del maní. Revista de la Facultad de Ciencias, 14(1), 23-38. https://doi.org/10.15446/rev.fac.cienc.v14n1.113747
Al-Karaki, G. N., & Othman, Y. (2023). Effect of foliar application of amino acid biostimulants on growth, macronutrient, total phenol contents and antioxidant activity of soilless grown lettuce cultivars. South African Journal of Botany, 154, 225-231. https://doi.org/10.1016/j.sajb.2023.01.034
Almutairi, K. F., Saleh, A. A., Moaaz Ali, M., Sas-Paszt, L., Abada, H. S., & Mosa, W. F. A. (2022). Growth performance of guava trees after the exogenous application of amino acids glutamic acid, arginine, and glycine. Horticulturae, 8(12), Artículo 111012. https://doi.org/10.3390/horticulturae8121110
Bakoulopoulou, I., Roussis, I., Kakabouki, I., Tigka, E., Stavropoulos, P., Mavroeidis, A., Karydogianni, S., Bilalis, D., & Papastylianou, P. (2025). Exploring phenological and agronomic parameters of Greek lentil landraces for developing climate-resilient cultivars adapted to Mediterranean conditions. Crops, 5(6), Artículo 91. https://doi.org/10.3390/CROPS5060091
Calero Hurtado, A., De Almeida, F. A. Q., da Silva Pinheiro, D. G., Botter Fasoli, J. V., da Silva Cabral, T. P., Wilke Rampassi, B., & Peña Calzada, K. (2026). The combined effects of plant density, chitosan, and amino acids enhance the sunflower growth and yield under water deficit conditions. AIMS Agriculture and Food, 11(2), 399–-421. https://doi.org/10.3934/agrfood.2026021
Calero Hurtado, A., Meléndrez Rodríguez, J. F., Peña Calzada, K., Pérez Díaz, Y., & Jiménez Medina, A. (2025). Foliar application of a mixture of amino acid-based growth promoters enhances tomato seedling production. Horticulturae, 11(6), Artículo 582. https://doi.org/10.3390/horticulturae11060582
Calero Hurtado, A., Peña Calzada, K., Fasoli, J. V. B., Jiménez, J., & Sánchez López, L. (2025). Synergic Effects of the microbial consortium and amino acid-based growth promoter in sunflower productivity under water-deficit conditions. Water, 17(9), Artículo 1365. https://doi.org/10.3390/w17091365
Calero Hurtado, A., Pérez Díaz, Y., Peña Calzada, K., & Meléndrez Rodríguez, J. F. (2025). Peanut growth and yield responses are influenced by plant density, microbial consortium inoculation, and amino acid application. Sustainability, 17(20), Artículo 9207. https://doi.org/10.3390/su17209207
Calero-Hurtado, A., Pérez-Díaz, Y., Hernández-González, L., García-Guardarrama, Y., Pacheco-Méndez, S. M., Rodríguez-Pérez, Y., & Castro-Lizazo, I. (2023). Coaplicación entre el consorcio microorganismos eficientes y Biobras-16® aumentan el crecimiento y la productividad del frijol común. Revista de la Facultad de Ciencias, 12(2), 64–-79. https://doi.org/10.15446/rev.fac.cienc.v12n2.107055
Calero-Hurtado, A., Pérez-Díaz, Y., Peña-Calzada, K., Jiménez-Medina, A., & Kukurtcu, B. (2025). Soybean responses to foliar amino acid application and high plant densities. Revista de Ciencias Agrícolas, 42(2), Artículo e2264. https://doi.org/10.22267/rcia.20254202.264
Calero-Hurtado, A., Pérez-Díaz, Y., Rodríguez-Lorenzo, M., & Rodríguez-González, V. (2022). Aplicación conjunta del consorcio microorganismos benéficos y FitoMas-E® incrementan los indicadores agronómicos del frijol. Revista U.D.C.A Actualidad & Divulgación Científica, 25(1), Artículo e2252. https://doi.org/10.31910/rudca.v25.n1.2022.2252
Du Jardin, P. (2015). Plant biostimulants: Definition, concept, main categories and regulation. Scientia Horticulturae, 196, 3-14. https://doi.org/10.1016/j.scienta.2015.09.021
El Sheikha, A. F., Allam, A. Y., Younes Taha, M., & Varzakas, T. (2022). How does the addition of biostimulants affect the growth, yield, and quality parameters of the snap bean (Phaseolus vulgaris L.)? How is this reflected in its nutritional value? Applied Sciences, 12(2), Artículo 776. https://doi.org/10.3390/app12020776
Henderson, B. C. R., Sanderson, J. M., & Fowles, A. (2025). A review of the foliar application of individual amino acids as biostimulants in plants. Discover Agriculture, 3, Artículo 69. https://doi.org/10.1007/s44279-025-00222-7
Jiang, Y., Yue, Y., Wang, Z., Lu, C., Yin, Z., Li, Y., & Ding, X. (2024). Plant biostimulant as an environmentally friendly alternative to modern agriculture. Journal of Agricultural and Food Chemistry, 72(10), 5107-5121. https://doi.org/10.1021/acs.jafc.3c09074
Kemp, C. D. (1960). Methods of estimating the leaf area of grasses from linear measurements. Annals of Botany, 24(4), 491-499. https://doi.org/10.1093/oxfordjournals.aob.a083723
Li, J., Van Gerrewey, T., & Geelen, D. (2022). A meta-analysis of biostimulant yield effectiveness in field trials. Frontiers in Plant Science, 13, Artículo 836702. https://doi.org/10.3389/fpls.2022.836702
Li, T., Chen, X., Cao, S., Liu, Z., Tian, L., Gao, Z., Sun, M., Zong, H., Wang, D., El-Sheikh, M. A., Yang, M., & Liu, P. (2024). Integrated physiological and transcriptome analyses of the effects of water-soluble amino acid fertilizer on plant growth. Journal of King Saud University - Science, 36(11), Artículo 103504. https://doi.org/10.1016/j.jksus.2024.103504
Ministerio de la Agricultura de Cuba. (2020). Instructivo técnico para el cultivo del frijol en Cuba (1.ª ed.).
Mosa, W. F. A., Ali, H. M., & Abdelsalam, N. R. (2021). The utilization of tryptophan and glycine amino acids as safe alternatives to chemical fertilizers in apple orchards. Environmental Science and Pollution Research, 28(2), 1983-1991. https://doi.org/10.1007/s11356-020-10658-7
Dias Nunes, H., Tiraboschi Leal, F., Checcio Mingotte, F. L., D’Amico Damião, V., Cuoto Junior, P. A., & Borges Lemos, L. (2021). Agronomic performance, quality and nitrogen use efficiency by common bean cultivars. Journal of Plant Nutrition, 44(7), 995-1009. https://doi.org/10.1080/01904167.2020.1849292
Peña Calzada, K., Calero Hurtado, A., Peistrup, V., Mühlmann, I., Rodríguez Miranda, D., Rodríguez Coca, L. I., Rodríguez González, M., & Rodríguez Fernández, J. C. (2024). Respuestas fisiológicas y productivas de plantas de remolacha tratadas con una solución de aminoácidos. Temas Agrarios, 29(1), 113-125. https://doi.org/10.21897/pkxmyw03
Peña Calzada, K., Olivera Viciedo, D., Habermann, E., Calero Hurtado, A., Lupino Gratão, P., De Mello Prado, R., Lata-Tenesaca, L. F., Martinez, C. A., Ajila Celi, G. E., & Rodríguez, J. C. (2022). Exogenous application of amino acids mitigates the deleterious effects of salt stress on soybean plants. Agronomy, 12(9), Artículo 2014. https://doi.org/10.3390/agronomy12092014
Peña-Calzada, K., Calero-Hurtado, A., Meléndrez-Rodríguez, J. F., Rodríguez-Fernández, J. C., Gutiérrez-Cádenas, O. G., García-González, M. T., Madrigal-Carmona, L., & Jiménez-Medina, A. (2025). Impacts of the biostimulant VIUSID® Agro on growth, productivity, and tolerance to salt stress in crops: A systematic review. Horticulturae, 11(4), Artículo 407. https://doi.org/10.3390/horticulturae11040407
Pérez Díaz, Y., Calero Hurtado, A., Peña Calzada, K., Gutiérrez Díaz, J. L., & Rodríguez González, V. (2024). Densidades de plantas y aplicación foliar de aminoácidos incrementan el rendimiento del ajonjolí. Temas Agrarios, 29(1), 100-112. https://doi.org/10.21897/w2sd1542
Pérez-Díaz, Y., Calero-Hurtado, A., Peña Calzada, K., & Jiménez Medina, A. (2025). Utilización de densidades de plantas y un consorcio microbiano para la producción sostenible de girasol. Agronomía Mesoamericana, 36, Artículo f9zbrr64. https://doi.org/10.15517/f9zbrr64
Pohlmann, V., Lago, I., Lopes, S. J., da Silva Martins, J. T., da Rosa, C. A., Caye, M., & Portalanza, D. (2021). Estimativa da área foliar de feijão comum (Phaseolus vulgaris) por método não destrutivo. Semina: Ciências Agrárias, 42(4), 2163-2180. https://doi.org/10.5433/1679-0359.2021v42n4p2163
IUSS Working Group WRB. (2022). World Reference Base for Soil Resources: International soil classification system for naming soils and creating legends for soil maps (4.ª ed.). International Union of Soil Sciences.
Zhang, Q., Liu, J., Jeong, S. J., Masabni, J., & Niu, G. (2025). Biostimulants applied in seedling stage can improve onion early bulb growth: Cultivar- and fertilizer-type-specific positive effects. Horticulturae, 11(4), Artículo 402. https://doi.org/10.3390/horticulturae11040402
Downloads
Published
License
Copyright (c) 2026 Jorge Félix Meléndrez Rodríguez, Alexander Calero Hurtado, Kolima Peña Calzada (Autor/a)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
