Morphological, morphometric and molecular identification of Meloidogyne exigua (Göeldi 1887) in coffee (Coffea arabica)
DOI:
https://doi.org/10.15517/am.v31i3.38733Keywords:
Coffea arabica, root-knot nematode, identification, molecular analysisAbstract
Introduction. The genus Meloidogyne contains many species and their correct diagnosis of these must be based on the use of multiple tools, which in diagnostic laboratories allow a correct and reliable evaluation, specially for quarantine nematodes. The proper identification of a phytonematode is important not only to establish an appropriate control strategy, but also to avoid its spread to other areas where it could cause damage that would result in economic losses. Objective. To perform taxonomic and molecular studies to confirm the Meloidogyne species, associated to a coffee plantation located in Barva, Heredia. Materials and methods. Soil and root samples were collected in a coffee plantation located in Barva, Heredia, Costa Rica, which were processed in the Nematology laboratory of the School of Agricultural Sciences at the Universidad Nacional (UNA), using the centrifugation-flotation method in sugary solution and molecular techniques. Results. On average, 30 J2 100 cc-1 were counted in soil and 1000 J2 10 g-1 of roots of the root-knot nematode. Taxonomic and molecular techniques allowed to identify that the females and juveniles extracted from coffee roots corresponded to Meloidogyne exigua. Analysis of the sequences generated by PCR and the use of the DraI enzyme confirmed the identity of the species with 99 % similarity when compared to sequences from Costa Rica and Nicaragua, reported in the Genbank. Conclusion. Using different morphometric and molecular techniques, M. exigua associated with a coffee plantation was identified.
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Adam, M.A.A., Phillips, M.S and V.C. Block. 2007. Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.). Plant Pathol. 56:190-197. DOI: https://doi.org/10.1111/j.1365-3059.2006.01455.x
Brito, J., T. Powers, P. Mullin, R. Inserra, and D. Dickson. 2004. Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida. J. Nematol. 36(3):232-240.
Cain, S.C. 1974. Meloidogyne exigua. C.I.H. descriptions of plant-parasitic nematodes. Set 4, # 49, Commonwealth Institute of Helminthology, Herts.
Carneiro, R.M.D.G., Tigano, M.S., Randig, O., Almeida, M.R.A.A and Sarah, J.L. 2004. Identification and genetic diversity of Meloidogyne spp. (Tylenchida: Meloidogynidae) on coffee from Brazil, Central America and Hawaii. Nematology 6(2):287-298. DOI: https://doi.org/10.1163/1568541041217942
Carneiro, R.M., and E.T. Cofcewicz. 2008. The taxonomy of Coffee-Parasitic root-knot nematodes, Meloidogyne spp. In: R.M. Souza, editor, Plant–parasitic nematodes of coffee. Springer, Dordrecht, NLD. p. 87-122. DOI: https://doi.org/10.1007/978-1-4020-8720-2_6
Castillo, J.H. 2014. Identificación de especies del género Meloidogyne spp. presentes en el municipio de Patzicía, Chimaltenango. Tesis de grado. Universidad Rafael Landívar, Nueva Guatemala de la Asunción, GUA.
Chitwood, B.G. 1949. Root-knot nematodes - Part 1. A revision of the genus Meloidogyne Göldi, 1887. Proc. Helminthol Soc Wash 16:90-104.
Darriba, D., G.L. Taboada, R. Doallo, and D. Posada. 2012. jModelTest 2: More models, new heuristics and parallel computing. Nature Methods 9:772-772. doi:10.1038/nmeth.2109. DOI: https://doi.org/10.1038/nmeth.2109
Edgar, R.C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32:1792-1797. doi:10.1093/nar/gkh340 DOI: https://doi.org/10.1093/nar/gkh340
Elling, A.A. 2013. Major emerging problems with minor Meloidogyne species. Phytopathology 103:1092-1102. doi:10.1094/PHYTO-01-13-0019-RVW DOI: https://doi.org/10.1094/PHYTO-01-13-0019-RVW
Esbenshade, P.R., and A.C. Triantaphyllou. 1990. Isozyme phenotypes for the identification of Meloidogyne species. J. Nematol. 22(1):10- 15.
Esser, R.P., V.G. Perry, and A.L. Taylor. 1976. A diagnostic compendium of the genus Meloidogyne (Nematoda: Heteroderidae). Proc. Helminthol. Soc. Wash. 43(2):138-150.
Ferreira, I y Crozzoli, R. 1995. Efectos del nematodo agallador Meloidogyne exigua sobre el crecimiento de plantas de café en vivero. Nematología Mediterránea 23:325-328.
Flores, L. 2008. Identificación de especies de Meloidogyne descritas en Costa Rica con base en la variabilidad del ADN mitocondrial. Tesis MSc., Universidad de Costa Rica, San José, CRI.
Fourie, H., Zijlstra, C. and McDonald, A.H. (2001) Identification of root-knot nematode species occurring in South Africa using the SCAR-PCR technique. Nematology 3:675-689. DOI: https://doi.org/10.1163/156854101753536046
Guindon, S., J.F. Dufayard, V. Lefort, M. Anisimova, W. Hordijk, and O. Gascuel. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59:307-321. doi:10.1093/sysbio/syq010 DOI: https://doi.org/10.1093/sysbio/syq010
Hall, T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98NT. Nucleic Acids Symp. Ser. 41:95-98.
Harris, T., L. Sandall, and T.O. Powers. 1990. Identification of single Meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial DNA. J. Nematol. 22:518-524.
Hartman, K., and J. Sasser. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal patterns morphology. In: K.R. Barker et al., editors, An advanced treatise on Meloidogyne biology and control. North Carolina State University Graphics, NC, USA. p. 69-77.
Hemmerle, W.J., and H.O. Hartley. 1973. Computing maximum likelihood estimates for the mixed AOV model using the W transformation. Technometrics 15:819-831. doi:10.2307/1267392 DOI: https://doi.org/10.1080/00401706.1973.10489115
Herrera, L., T. Bryngelsson, A. Monzón, and M. Geleta. 2011. Identification of root-knot based on perineal pattern, SCAR markers and nuclear ribosomal DNA sequences. Nematol. Mediterr. 39(2):101-110.
Holdridge, L. 1982. Ecología basada en zona de vida. Instituto Interamericano de Cooperación para la Agricultura, San José, CRI.
Humphreys-Pereira D.A., L. Flores-Chaves, M. Gómez, L. Salazar, L. Gómez-Alpízar, and A.A. Elling. 2014. Meloidogyne lopezi sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with coffee (Coffea arabica L.) in Costa Rica, its diagnosis and phylogenetic relationship with other coffee-parasitizing Meloidogyne species. Nematology 16:643-61. doi:10.1163/15685411-00002794 DOI: https://doi.org/10.1163/15685411-00002794
Jeyaprakash, A., M. Tigano, J. Brito, R. Carneiro, and D. Dickson. 2006. Differentiation of Meloidogyne floridensis from M. arenaria using high-fidelity PCR amplified mitochondrial AT-rich sequences. Nematropica 36:1-12.
Kepenekçi İ., E. Evlice, G. Öztürk. 2014. Taxonomic characteristics of Meloidogyne exigua Goeldi which is a new root-knot nematode for Turkey and other root-knot nematode species. Bitki Koruma Bülteni 54:1-9.
Long, H.B., Y.F. Sun, T.Z. Feng, Y.L. Pei, and D.L Peng. 2017. First Report of Meloidogyne graminicola on Soybean (Glycine max) in China. Plant Dis. 101(8):1554. doi:10.1094/PDIS-03-17-0334-PDN DOI: https://doi.org/10.1094/PDIS-03-17-0334-PDN
López, R. 1984. Differential plants responses and morphometrics of some Meloidogyne spp. from Costa Rica. Turrialba 34:445-458.
López, R., y D. Dickson. 1977. Morfometría y respuesta de hospedantes diferenciales a tres poblaciones de Meloidogyne incognita y una de Meloidogyne javanica. Agron. Costarricense 1(2):119-127.
López, R., y H. Vílchez. 1991. Dos nuevos hospedantes de Meloidogyne exigua en Costa Rica. Agron. Costarricense 15 (1/2):163-166.
López, R., y L. Salazar. 1989. Meloidogyne arabicida sp. (Nematoda: Heteroderidae) nativo de Costa Rica: un nuevo y severo patógeno del cafeto. Turrialba 39:313-323.
Lordello, L.G., and A.P. Zamith. 1958. On the morphology of the coffee root-knot nematode, Meloidogyne exigua Goeldi, 1887. Helminthol. Soc. 25(2):133-137.
Luc, M., R.A. Sikora, and J. Bridge. 1990. Plant parasitic nematodes in subtropical and tropical agriculture. CAB International, Wallingford, GBR.
McClure, M.A., C. Nischwitz, AM. Skantar, ME. Schmitt, and S.A. Subbotin. 2012. Root-knot nematodes in golf course greens of the western United States. Plant Dis. 96:635-647. doi:10.1094/PDIS-09-11-0808 DOI: https://doi.org/10.1094/PDIS-09-11-0808
Orui, Y. 1998. Identification of Japanese species of the genus Meloidogyne (nematode: Meloidogynidae) by PCR-RFLP analysis. Appl. Entomol. Zool. 33:43-51. doi:10.1303/aez.33.43 DOI: https://doi.org/10.1303/aez.33.43
Peraza, W., Rosales, J., Esquivel, A., Hilje, I., Molina, y R., Castillo. 2013. Identificación morfológica, morfométrica y molecular de Meloidogyne incognita en higuera (Ficus carica L.) en Costa Rica. Agron. Mesoam. 24(2):337-346. doi:10.15517/am.v24i2.12533 DOI: https://doi.org/10.15517/am.v24i2.12533
Powers, T., and T. Harris. 1993. A polymerase chain reaction method of identification five major Meloidogyne species. J. Nematol. 25(1):1-6.
Powers, T.O., P.G. Mullin, T.S. Harris, L.A. Sutton, and R.S. Higgins. 2005. Incorporating molecular identification of Meloidogyne spp. into a largescale regional nematode survey. J. Nematol. 37:226-235.
Randig, O., M. Bongiovanni, R., Carneiro, and P. Castagnone-Sereno. 2002. Genetic diversity of root-knot nematodes from Brazil and development of SCAR marker specific for the coffee damaging species. Genome 45:862-870. DOI: https://doi.org/10.1139/g02-054
Rodríguez-Da-silva, L. 2011. Efeito do extracto de capeba Pothomorphe umbellata (L.) MIQ. Na mobilidade de juvenis de segundo estádio de Meloidogyne exigua. Tesis Grado, Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais, Muzambinho, BRA.
Rojas, M. 2008. Avance sobre la caracterización morfológica y molecular de especies de Meloidogyne en cafetales de Costa Rica. Instituto del Café de Costa Rica, Heredia, CRI.
Rojas, M., y L. Salazar. 2013. Densidad crítica de Meloidogyne exigua en plantas de almácigo de café variedad Caturra. Agron. Costarricense 37(2):115-123.
Salgado, M., L. Oliveira, R. Silva, y V. Campos. 2008. Aspectos importantes de los fitonematodos del cafeto. Informe agrícola 29(247):42-50.
Sasser, J.N. 1979. Economic importance of Meloidogyne in tropical countries. In: F. Lamberti, and C.E. Taylor, editors, Root-knot nematodes. Academic Press, London, GBR. p. 360-374.
Solano, S., A. Esquivel, R. Molina, y B. Morera. 2015. Identificación de especies de Meloidogyne asociadas a plantas ornamentales de altura en Costa Rica. Agron. Mesoam. 26:247-256. doi:10.15517/am.v26i2.19280 DOI: https://doi.org/10.15517/am.v26i2.19280
Solano, F., Beacham, J., Thomas, S., Steinberger, y Hanson, S. 2013. Evaluación de la biodiversidad de nematodos del suelo en muestras de suelo conservadas. Nematodos En el Congreso de la Sociedad Americana de Fitopatólogos (2013APS Anual, Austin, Texas). La Sociedad Americana de Fitopatología, APS. Reunión conjunta de MSA. Austin, Texas, USA.
Taylor, A., and W.Q. Loegering. 1953. Nematodes associated with roots lesions in ábaca. Turrialba 3:8-13.
Taylor, A., y J. Sasser. 1983. Biología, identificación y control de los nematodos del nudo de la raíz: (Especies de Meloidogyne). North Carolina State University Graphics, NC, USA.
Villain, L., J.L. Sarah, A. Hernández, B. Bertrand, F. Anthony, P. Lashermes, P. Charmetant, F. Anzueto, P. Figueroa, and R.M.D.G. Carneiro. 2013. Diversity of root-knot nematodes associated with coffee orchards in Central America. Nematropica 43:194-206.
Whitehead, A. 1968. Taxonomy of Meloidogyne (Nematodea: Heteroderidae) with descriptions of four new species. Trans. Zool. Soc. Lond. 31:263-401. doi:10.1111/j.1096-3642.1968.tb00368.x DOI: https://doi.org/10.1111/j.1096-3642.1968.tb00368.x
World Coffee Research (WCR). 2016. Las variedades de café de Mesoamérica y El Caribe, WCR, TX, USA. https://worldcoffeeresearch.org/documents/26/Variedades_de_Cafe_de_Mesoamerica_y_el_Caribe_20160609.pdf (consultado 25 ago. 2016).
Xu, J., P. Lui, Q. Meng, and H. Long. 2004. Characterization of Meloidogyne species from China using isoenzyme, phenotypes and amplified mitochondrial DNA restriction fragments length polymorphism. Eur. J. Plant Phathol. 110:309-315. doi:10.1023/B:EJPP.0000019800.47389.31 DOI: https://doi.org/10.1023/B:EJPP.0000019800.47389.31
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