Antioxidant enzyme activity of ripe and unripe pulp of Vasconcellea candicans (A. Gray) A. DC 1864 (Caricaceae)
DOI:
https://doi.org/10.15517/4m2rxg37Keywords:
Catalase, peroxidase, superoxide dismutase, glutathione sulfhydryl transferase, mito fruit, andean papayaAbstract
Introduction. The determination of antioxidant components of native fruits is important for their incorporation into the diet and for their better use and conservation. Objective. To evaluate the activity of the antioxidant enzymes catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) and glutathione sulfhydril transferase (GST), of aqueous extracts of ripe and unripe pulp of the fruit of Vasconcellea candicans (A. Gray) A. DC (“mito”, “andean papaya”) and relate them to their concentration of total polyphenols (PT). Materials and methods. The pulp of four fruits, ripe and unripe, of “mito” from Santo Domingo de Los Olleros, Huarochirí, Lima, Peru, was extracted and processed during March to December 2023. The samples were obtained by aqueous extraction of 20 g of pulp in a 20:80 ratio in water at 8 °C for 15 days (cold maceration technique), using the supernatant after centrifugation. The determination of PT was performed by spectrophotometry using gallic acid as standard. The determination of the specific activity of the enzymes was performed by spectrophotometry using the respective standards. Non-parametric statistics were applied using the statistical software R. Results. The fruits of V. candicans show marked differences between stages of ripeness, particularly in protein content and in the activity of antioxidant enzymes such as CAT, SOD and GST. Biochemical correlations are more pronounced in ripe fruits, indicating a functional reorganization of antioxidant components during ripening. Conclusions. The results suggest that CAT activity, together with polyphenol levels, could be used as a biochemical indicator of the degree of fruit ripeness, which could be used to determine and optimize the post-harvest shelf life of the fruit.
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