Isolation of rumen cellulolytic bacterial consortia and in vitro simulation of rumen microbiota manipulation
DOI:
https://doi.org/10.15517/am.2025.62973Keywords:
biogas, degradation, enzyme activity, gas production, rumenAbstract
Introduction. The isolation and manipulation of rumen cellulolytic bacterial consortia (CBC) enhance fiber degradation under in vitro conditions. Objective. To obtain CBC from different substrates and simulate ruminal microbiota (RM) manipulation through in vitro gas production test. Materials and methods. The study was conducted from January to June 2024 at the Autonomous University of Guerrero, Cuajinicuilapa, Guerrero, Mexico. CBC were
obtained from rumen fluid using selective culture media with three distinct substrates: ground sawdust (CBCa),
Mulatto grass stalk (CBCt), and ground Mulatto grass (CBCm). Two in vitro assays were performed: a) evaluation of the CBC obtained, and b) addition of the CBC to RM. Both assays measured partial biogas production at intervals 0 to 24 h, 24 to 48 h, and 48 to 72 h, and cumulative production. Dry matter degradation (DMD) and cellulolytic enzyme activity (CEA) were assessed at 24, 48, and 72 h. Variables were analyzed in a completely randomized design. Results. In trial 1, CBCa demonstrated superior cumulative biogas production (p < 0.05); DMD at 24 and 72 h was higher for CBCa (p < 0.05); DMD at 48 h and CEA showed no significant differences among CBC (p > 0.05). In trial 2, the addition of CBC to RM did not significantly alter partial biogas production, DMD at 24, 48, and 72 h, nor CEA at 24 and 48 h (p > 0.05). However, the addition of CBCm increased 13.8 and 36.3 % the cumulative biogas production and CEA at 72 h, respectively. Conclusion. CBC obtained from Mulatto grass demonstrated potential for
RM manipulation under the specific in vitro conditions of this study.
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