An overview on extenders used in ram sperm cryopreservation
DOI:
https://doi.org/10.15517/am.2024.59591Keywords:
Freezing, semen extenders, sperm quality, animal reproduction, cryobiologyAbstract
Introduction. One of the most critical methods for improving domestic animal reproduction is sperm cryopreservation. To achieve the best results, this complex process requires the meticulous balance of numerous variables. Sperm cryopreservation is an essential technique in programs that improve breeding and conservation for many species, especially small ruminants. In this case, genetic material from a limited number of exceptional sires can be used to breed a large number of female sheep. Objective. To analyze the current advances in extenders used in ram sperm cryopreservation. Development. In rams, spermatozoa have a lower intramembrane cholesterol-to-phospholipid ratio than other species, making them more susceptible to cryopreservation damage than other livestock species. Adequate freezing and thawing can prevent metabolic and structural damage to ram sperm cells, a goal that has not yet been achieved. The success of the sperm freezing process depends on several factors, such as freezing/thawing rate, equilibration time, spermatozoa, and the composition of medium surrounding spermatozoa, among others. In this sense, sperm extender is one of the most critical factors in the cryopreservation process. Conclusions. The vulnerabilities of ram sperm to cryogenic stress poses challenges to maintaining viability after thawing and achieving consistently high fertility rates. Further research to refine supplementation strategies, optimize freezing protocols, and explore novel cryoprotectants is essential to overcome these challenges and enhance the efficacy of ram semen cryopreservation to improve reproductive outcomes in ovine breeding programs.
Downloads
References
Abdelnour, S. A., Abd El-Hack, M. E., Alagawany, M., Taha, A. E., Elnesr, S. S., Abd Elmonem, O. M., & Swelum, A. A. (2020). Useful impacts of royal jelly on reproductive sides, fertility rate and sperm traits of animals. Journal of Animal Physiology and Animal Nutrition, 104(6), 1798–1808. https://doi.org/10.1111/jpn.13303 DOI: https://doi.org/10.1111/jpn.13303
Aisen, E. G., Medina, V. H., & Venturino, A. (2002). Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57(7), 1801–1808. https://doi.org/10.1016/S0093-691X(02)00653-2 DOI: https://doi.org/10.1016/S0093-691X(02)00653-2
Aisen, E., Quintana, M., Medina, V., Morello, H., & Venturino, A. (2005). Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose-based hypertonic extenders. Cryobiology, 50(3), 239–249. https://doi.org/10.1016/j.cryobiol.2005.02.002 DOI: https://doi.org/10.1016/j.cryobiol.2005.02.002
Alcay, S., Berk Toker, M., Gokce, E., Ustuner, B., Tekin Onder, N., Sagirkaya, H., Nur, Z., & Kemal Soylu, M. (2015). Successful ram semen cryopreservation with lyophilized egg yolk-based extender. Cryobiology, 71(2), 329–333. https://doi.org/10.1016/j.cryobiol.2015.08.008 DOI: https://doi.org/10.1016/j.cryobiol.2015.08.008
Allai, L., Benmoula, A., Marciane da Silva, M., Nasser, B., & El Amiri, B. (2018). Supplementation of ram semen extender to improve seminal quality and fertility rate. Animal Reproduction Science, 192, 6–17. https://doi.org/10.1016/j.anireprosci.2018.03.019 DOI: https://doi.org/10.1016/j.anireprosci.2018.03.019
Allai, L., Druart, X., Louanjli, N., Contell, J., Nasser, B., & El Amiri, B. (2017). Improvements of ram semen quality using cactus seed oil during liquid preservation in Tris egg yolk and skim milk based extenders. Small Ruminant Research, 151, 16–21. https://doi.org/10.1016/j.smallrumres.2017.02.001 DOI: https://doi.org/10.1016/j.smallrumres.2017.02.001
Allai, L., Druart, X., Öztürk, M., BenMoula, A., Nasser, B., & El Amiri, B. (2016). Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage. Animal Reproduction Science, 175, 1–9. https://doi.org/10.1016/j.anireprosci.2016.09.013 DOI: https://doi.org/10.1016/j.anireprosci.2016.09.013
Allai, L., Druart, X., Terzioğlu, P., Louanjli, N., Nasser, B., Ozturk, M., & El Amiri, B. (2023). The addition of Opuntia ficus-indica ethanolic extract to a skimmed milk-based extender impacts ram sperm quality. Veterinary Medicine International, 2023(1), Article 6248890. https://doi.org/10.1155/2023/6248890 DOI: https://doi.org/10.1155/2023/6248890
Amidi, F., Pazhohan, A., Nashtaei, M. S., Khodarahmian, M., & Nekoonam, S. (2016). The role of antioxidants in sperm freezing: a review. Cell and Tissue Banking, 17, 745–756. https://doi.org/10.1007/S10561-016-9566-5 DOI: https://doi.org/10.1007/s10561-016-9566-5
Asadzadeh, N., Abdollahi, Z., Esmaeilkhanian, S., & Masoudi, R. (2021). Fertility and flow cytometry evaluations of ram frozen semen in plant-based extender supplemented with Mito-TEMPO. Animal Reproduction Science, 233, Article 106836. https://doi.org/10.1016/j.anireprosci.2021.106836 DOI: https://doi.org/10.1016/j.anireprosci.2021.106836
Barbas, J. P., Pimenta, J., Baptista, M. C., Marques, C. C., Pereira, R. M. L. N., Carolino, N., & Simões, J. (2023). Ram semen cryopreservation for Portuguese native breeds: Season and breed effects on semen quality variation. Animals, 13(4), Article 579. https://doi.org/10.3390/ani13040579 DOI: https://doi.org/10.3390/ani13040579
Batissaco, L., Paes de Arruda, R., Rodrigues Alves, M. B., Andrade Torres, M., Menegon Lemes, K., Romano Prado-Filho, R., Guibu de Almeida, T., De Andrade, A. F. C., & Carvalho Celeghini, E. C. (2020). Cholesterol-loaded cyclodextrin is efficient in preserving sperm quality of cryopreserved ram semen with low freezability. Reproductive Biology, 20(1), 14–24. https://doi.org/10.1016/j.repbio.2020.01.002 DOI: https://doi.org/10.1016/j.repbio.2020.01.002
Ben Moula, A., Rahim, A., Chentouf, M., Raes, M., Kirschvink, N., & El Amiri, B. (2023). Inclusion of Spirulina platensis and Salvia verbenaca extracts to boost semen quality and fertilization ability in sheep. Reproduction in Domestic Animals, 58(5), 637–645. https://doi.org/10.1111/rda.14332 DOI: https://doi.org/10.1111/rda.14332
Benko, F., Mohammadi-Sangcheshmeh, A., Ďuračka, M., Lukáč, N., & Tvrdá, E. (2022). In vitro versus cryo-induced capacitation of bovine spermatozoa, part 1: structural, functional, and oxidative similarities and differences. PLoS ONE, 17(10), Article e0276683. https://doi.org/10.1371/journal.pone.0276683 DOI: https://doi.org/10.1371/journal.pone.0276683
Benson, J. D., Woods, E. J., Walters, E. M., & Critser, J. K. (2012). The cryobiology of spermatozoa. Theriogenology, 78(8), 1682–1699. https://doi.org/10.1016/j.theriogenology.2012.06.007 DOI: https://doi.org/10.1016/j.theriogenology.2012.06.007
Bergeron, A., Brindle, Y., Blondin, P., & Manjunath, P. (2007). Milk caseins decrease the binding of the major bovine seminal plasma proteins to sperm and prevent lipid loss from the sperm membrane during sperm storage. Biology of Reproduction, 77(1), 120–126. https://doi.org/10.1095/biolreprod.106.058248 DOI: https://doi.org/10.1095/biolreprod.106.058248
Bergeron, A., & Manjunath, P. (2006). New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molecular Reproduction and Development, 73(10), 1338–1344. https://doi.org/10.1002/mrd.20565 DOI: https://doi.org/10.1002/mrd.20565
Chen, Y., Meng, F., Liu, Y., Zhu, C., Ling, Y., Liu, C., Li, L., Liu, Y., He, X., Cao, J., & Zhang, Y. (2024). Effects of resveratrol on DLD and NDUFB9 decrease in frozen semen of Mongolian sheep. Cryobiology, 114, Article 104791. https://doi.org/10.1016/j.cryobiol.2023.104791 DOI: https://doi.org/10.1016/j.cryobiol.2023.104791
Colas, G. (1975). Effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep-frozen ram semen. Reproduction, 42(2), 277–285. https://doi.org/10.1530/jrf.0.0420277 DOI: https://doi.org/10.1530/jrf.0.0420277
Cseh, S., Faigl, V., & Amiridis, G. S. (2012). Semen processing and artificial insemination in health management of small ruminants. Animal Reproduction Science, 130(3-4), 187–192. https://doi.org/10.1016/j.anireprosci.2012.01.014 DOI: https://doi.org/10.1016/j.anireprosci.2012.01.014
Del Valle, I., Souter, A., Maxwell, W. M. C., Muiño-Blanco, T., & Cebrián-Pérez, J. A. (2013). Function of ram spermatozoa frozen in diluents supplemented with casein and vegetable oils. Animal Reproduction Science, 138(3-4), 213–219. https://doi.org/10.1016/j.anireprosci.2013.02.022 DOI: https://doi.org/10.1016/j.anireprosci.2013.02.022
El-Seadawy, I. E., Kotp, M. S., El-Maaty, A. M. A., Fadl, A. M., El-Sherbiny, H. R., & Abdelnaby, E. A. (2022). The impact of varying doses of moringa leaf methanolic extract supplementation in the cryopreservation media on sperm quality, oxidants, and antioxidant capacity of frozen-thawed ram sperm. Tropical Animal Health and Production, 54(6), Article 344. https://doi.org/10.1007/s11250-022-03344-y DOI: https://doi.org/10.1007/s11250-022-03344-y
Emamverdi, M., Zhandi, M., Zare Shahneh, A., Sharafi, M., & Akbari-Sharif, A. (2013). Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender. Reproduction in Domestic Animals, 48(6), 899–904. https://doi.org/10.1111/rda.12183 DOI: https://doi.org/10.1111/rda.12183
Forouzanfar, M., Sharafi, M., Hosseini, S. M., Ostadhosseini, S., Hajian, M., Hosseini, L., Abedi, P., Nili, N., Rahmani, H. R., & Nasr-Esfahani, M. H. (2010). In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen. Theriogenology, 73(4), 480–487. https://doi.org/10.1016/j.theriogenology.2009.10.005 DOI: https://doi.org/10.1016/j.theriogenology.2009.10.005
Freitas Bittencourt, R., Oba, E., De Almeida Biscarde, C. E., Costa Azevedo, H., Vasconcelos Bittencourt, M., Oliveira de Menezes, G. F., Da Silva Lima, A., Da Mata Fuchs, K., & De Lisboa Ribeiro Filho, A. (2018). Dimethylacetamide and trehalose for ram semen cryopreservation. Cryobiology, 85, 1–6. https://doi.org/10.1016/j.cryobiol.2018.10.266 DOI: https://doi.org/10.1016/j.cryobiol.2018.10.266
Gil, J., Lundeheim, N., Söderquist, L., & Rodrı́guez-Martı́nez, H. (2003). Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen. Theriogenology, 59(5-6), 1241–1255. https://doi.org/10.1016/S0093-691X(02)01177-9 DOI: https://doi.org/10.1016/S0093-691X(02)01177-9
Gosálvez, J., López-Fernández, C., Fernández, J. L., Gouraud, A., & Holt, W. V. (2011). Relationships between the dynamics of iatrogenic DNA damage and genomic design in mammalian spermatozoa from eleven species. Molecular Reproduction and Development, 78(12), 951–961. https://doi.org/10.1002/MRD.21394 DOI: https://doi.org/10.1002/mrd.21394
Jihad Neamah, H. (2022). Evaluation of adding prickly pear extracts to the diluted ram’s semen at preservation. Journal of Animal Health and Production, 11(1), 94–98. https://doi.org/10.17582/journal.jahp/2023/11.1.94.98 DOI: https://doi.org/10.17582/journal.jahp/2023/11.1.94.98
Keskin, N., Erdogan, C., Bucak, M. N., Ozturk, A. E., Bodu, M., Ili, P., Baspinar, N., & Dursun, S. (2020). Cryopreservation effects on ram sperm ultrastructure. Biopreservation and Biobanking, 18(5), 441–448. https://doi.org/10.1089/bio.2020.0056 DOI: https://doi.org/10.1089/bio.2020.0056
Kumar Jha, P., Shahi Alam, M. G., Mansur, A. A., Naher, N., Islam, T., Uddin Bhuiyan, M., & Bari, F. Y. (2019). Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques. Cryobiology, 89, 35–41. https://doi.org/10.1016/j.cryobiol.2019.06.001 DOI: https://doi.org/10.1016/j.cryobiol.2019.06.001
Layek, S. S., Mohanty, T. K., Kumaresan, A., & Parks, J. E. (2016). Cryopreservation of bull semen: Evolution from egg yolk based to soybean based extenders. Animal Reproduction Science, 172, 1–9. https://doi.org/10.1016/j.anireprosci.2016.04.013 DOI: https://doi.org/10.1016/j.anireprosci.2016.04.013
Ledesma, A., Zalazar, L., Buchelly Imbachi, F., Pastore, J. I., Brown, P., Eddy, E. M., Hozbor, F., & Cesari, A. (2019). Recombinant peptide reverses cryo-capacitation in ram sperm and improves in vitro fertilization. Animal Reproduction Science, 207, 61–72. https://doi.org/10.1016/j.anireprosci.2019.05.016 DOI: https://doi.org/10.1016/j.anireprosci.2019.05.016
Liu, G., Pan, B., Li, S., Ren, J., Wang, B., Wang, C., Su, X., & Dai, Y. (2020). Effect of bioactive peptide on ram semen cryopreservation. Cryobiology, 97, 153–158. https://doi.org/10.1016/j.cryobiol.2020.08.007 DOI: https://doi.org/10.1016/j.cryobiol.2020.08.007
Luna-Orozco, J. R., González-Ramos, M. A., Calderón-Leyva, G., Gaytán-Alemán, L. R., Arellano-Rodríguez, F., Ángel-García, O., & Véliz-Deras, F. G. (2019). Comparison of different diluents based on liposomes and egg yolk for ram semen cooling and cryopreservation. Iranian Journal of Veterinary Research, 20(2), 126–130. https://doi.org/10.22099/ijvr.2019.5262
Marcantonini, G., Bartolini, D., Zatini, L., Costa, S., Passerini, M., Rende, M., Luca, G., Basta, G., Murdolo, G., Calafiore, R., & Galli, F. (2022). Natural cryoprotective and cytoprotective agents in cryopreservation: a focus on melatonin. Molecules, 27(10), Article 3254. https://doi.org/10.3390/molecules27103254 DOI: https://doi.org/10.3390/molecules27103254
Masoudi, R., Sharafi, M., & Shahneh, A. Z. (2019). Effects of CoQ10 on the quality of ram sperm during cryopreservation in plant and animal based extenders. Animal Reproduction Science, 208, Article 106103. https://doi.org/10.1016/j.anireprosci.2019.06.015 DOI: https://doi.org/10.1016/j.anireprosci.2019.06.015
Mehdipour, M., Daghigh Kia, H., Najafi, A., Vaseghi Dodaran, H., & García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3), 297–303. https://doi.org/10.1016/j.cryobiol.2016.10.008 DOI: https://doi.org/10.1016/j.cryobiol.2016.10.008
Mehdipour, M., Daghigh Kia, H., Nazari, M., & Najafi, A. (2017). Effect of lecithin nanoliposome or soybean lecithin supplemented by pomegranate extract on post-thaw flow cytometric, microscopic and oxidative parameters in ram semen. Cryobiology, 78, 34–40. https://doi.org/10.1016/j.cryobiol.2017.07.005 DOI: https://doi.org/10.1016/j.cryobiol.2017.07.005
Moradi, M., Hajarian, H., Karamishabankareh, H., Soltani, L., & Soleymani, B. (2022). Pre-treatment of ram semen extender with magnetic nanoparticles on freeze-thawed spermatozoa. Veterinary Medicine and Science, 8(2), 792–798. https://doi.org/10.1002/vms3.689 DOI: https://doi.org/10.1002/vms3.689
Motlagh, M. K., Sharafi, M., Zhandi, M., Mohammadi-Sangcheshmeh, A., Shakeri, M., Soleimani, M., & Zeinoaldini, S. (2014). Antioxidant effect of rosemary (Rosmarinus officinalis L.) extract in soybean lecithin-based semen extender following freeze–thawing process of ram sperm. Cryobiology, 69(2), 217–222. https://doi.org/10.1016/j.cryobiol.2014.07.007 DOI: https://doi.org/10.1016/j.cryobiol.2014.07.007
Murawski, M., Schwarz, T., Grygier, J., Patkowski, K., Oszczęda, Z., Jelkin, I., Kosiek, A., Gruszecki, T. M., Szymanowska, A., Skrzypek, T., Zieba, D. A., & Bartlewski, P. M. (2015). The utility of nanowater for ram semen cryopreservation. Experimental Biology and Medicine, 240(5), 611–617. https://doi.org/10.1177/1535370214557219 DOI: https://doi.org/10.1177/1535370214557219
Najafi, A., Daghigh-Kia, H., Dodaran, H. V., Mehdipour, M., & Alvarez-Rodriguez, M. (2017). Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation. Animal Reproduction Science, 177, 35–41. https://doi.org/10.1016/j.anireprosci.2016.12.004 DOI: https://doi.org/10.1016/j.anireprosci.2016.12.004
Najafi, A., Najafi, M., Zanganeh, Z., Sharafi, M., Martinez-Pastor, F., & Adeldust, H. (2014). Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant. Reproduction in Domestic Animals, 49(6), 934–940. https://doi.org/10.1111/rda.12405 DOI: https://doi.org/10.1111/rda.12405
Najafi, A., Zhandi, M., Towhidi, A., Sharafi, M., Akbari Sharif, A., Khodaei Motlagh, M., & Martinez-Pastor, F. (2013). Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 66(3), 275–282. https://doi.org/10.1016/j.cryobiol.2013.03.002 DOI: https://doi.org/10.1016/j.cryobiol.2013.03.002
Ofosu, J., Qazi, I. H., Fang, Y., & Zhou, G. (2021). Use of melatonin in sperm cryopreservation of farm animals: a brief review. Animal Reproduction Science, 233, Article 106850. https://doi.org/10.1016/j.anireprosci.2021.106850 DOI: https://doi.org/10.1016/j.anireprosci.2021.106850
Paul, R. K., Kumar, D., & Singh, R. (2021). Carboxymethyl cellulose and glycerol act synergistically as cryoprotectant during cryopreservation of ram semen. Cryobiology, 101, 61–66. https://doi.org/10.1016/j.cryobiol.2021.06.001 DOI: https://doi.org/10.1016/j.cryobiol.2021.06.001
Peris-Frau, P., Martín-Maestro, A., Iniesta-Cuerda, M., Sánchez-Ajofrín, I., Cesari, A., Garde, J. J., Villar, M., & Soler, A. J. (2020). Cryopreservation of ram sperm alters the dynamic changes associated with in vitro capacitation. Theriogenology, 145, 100–108. https://doi.org/10.1016/j.theriogenology.2020.01.046 DOI: https://doi.org/10.1016/j.theriogenology.2020.01.046
Pini, T., Rickard, J. P., Leahy, T., Crossett, B., Druart, X., & De Graaf, S. P. (2018). Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa. Journal of Proteomics, 181, 73–82. https://doi.org/10.1016/j.jprot.2018.04.001 DOI: https://doi.org/10.1016/j.jprot.2018.04.001
Plante, G., Lusignan, M.-F., Lafleur, M., & Manjunath, P. (2015). Interaction of milk proteins and binder of sperm (BSP) proteins from boar, stallion and ram semen. Reproductive Biology and Endocrinology, 13(1), Article 92. https://doi.org/10.1186/s12958-015-0093-1 DOI: https://doi.org/10.1186/s12958-015-0093-1
Pool, K. R., Rickard, J. P., & De Graaf, S. P. (2021). Melatonin improves the motility and DNA integrity of frozen-thawed ram spermatozoa likely via suppression of mitochondrial superoxide production. Domestic Animal Endocrinology, 74, Article 106516. https://doi.org/10.1016/j.domaniend.2020.106516 DOI: https://doi.org/10.1016/j.domaniend.2020.106516
Ramírez-Vasquez, R. R. A., Cano, A., Hozbor, F. A., & Cesari, A. (2019). Cryopreservation and egg yolk extender components modify the interaction between seminal plasma proteins and the sperm surface. Theriogenology, 140, 153–163. https://doi.org/10.1016/j.theriogenology.2019.08.025 DOI: https://doi.org/10.1016/j.theriogenology.2019.08.025
Ramírez-Vasquez, R., Cesari, A., Greco, M. B., Cano, A., & Hozbor, F. (2019). Extenders modify the seminal plasma ability to minimize freeze-thaw damage on ram sperm. Reproduction in Domestic Animals, 54(12), 1621–1629. https://doi.org/10.1111/rda.13571 DOI: https://doi.org/10.1111/rda.13571
Riesco, M. F., Alvarez, M., Anel-Lopez, L., Neila-Montero, M., Palacin-Martinez, C., Montes-Garrido, R., Boixo, J. C., De Paz, P., & Anel, L. (2021). Multiparametric study of antioxidant effect on ram sperm cryopreservation—from field trials to research bench. Animals, 11(2), Article 283. https://doi.org/10.3390/ani11020283 DOI: https://doi.org/10.3390/ani11020283
Rostami, B., Ebrahimi, D., Sadeghipanah, H., Masoumi, R., & Shahir, M. H. (2020). Effects of supplementation of tris-egg yolk extender with different sugars and antioxidants on freezability of ram semen. Cryobiology, 92, 62–66. https://doi.org/10.1016/j.cryobiol.2019.10.198 DOI: https://doi.org/10.1016/j.cryobiol.2019.10.198
Saha, A., Asaduzzaman, M., & Bari, F. Y. (2022). Cryopreservation techniques for ram sperm. Veterinary Medicine International, 2022(1), Article 7378379. https://doi.org/10.1155/2022/7378379 DOI: https://doi.org/10.1155/2022/7378379
Salamon, S., & Maxwell, W. M. C. (2000). Storage of ram semen. Animal Reproduction Science, 62(1–3), 77–111. https://doi.org/10.1016/S0378-4320(00)00155-X DOI: https://doi.org/10.1016/S0378-4320(00)00155-X
Sarıözkan, S., Bucak, M. N., Tuncer, P. B., Ulutaş, P. A., & Bilgen, A. (2009). The influence of cysteine and taurine on microscopic–oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology, 58(2), 134–138. https://doi.org/10.1016/j.cryobiol.2008.11.006 DOI: https://doi.org/10.1016/j.cryobiol.2008.11.006
Sharafi, M., Borghei-Rad, S. M., Hezavehei, M., Shahverdi, A., & Benson, J. D. (2022). Cryopreservation of semen in domestic animals: a review of current challenges, applications, and prospective strategies. Animals, 12(23), Article 3271. https://doi.org/10.3390/ani12233271 DOI: https://doi.org/10.3390/ani12233271
Silvestre, M. A., Yániz, J. L., Peña, F. J., Santolaria, P., & Castelló-Ruiz, M. (2021). Role of antioxidants in cooled liquid storage of mammal spermatozoa. Antioxidants, 10(7), Article 1096. https://doi.org/10.3390/antiox10071096 DOI: https://doi.org/10.3390/antiox10071096
Sobeh, M., Hassan, S. A., Hassan, M. A. E., Khalil, W. A., Abdelfattah, M. A. O., Wink, M., & Yasri, A. (2020). A polyphenol-rich extract from Entada abyssinica reduces oxidative damage in cryopreserved ram semen. Frontiers in Veterinary Science, 7, Article 604477. https://doi.org/10.3389/fvets.2020.604477 DOI: https://doi.org/10.3389/fvets.2020.604477
Toker, M. B., Alcay, S., Gokce, E., & Ustuner, B. (2016). Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology, 72(3), 205–209. https://doi.org/10.1016/j.cryobiol.2016.05.001 DOI: https://doi.org/10.1016/j.cryobiol.2016.05.001
Üstüner, B., Alçay, S., Nur, Z., Sağirkaya, H., & Soylu, M. K. (2014). Effect of egg yolk and soybean lecithin on Tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Universitesi Veteriner Fakultesi Dergisi, 20(3), 393-398. https://scispace.com/pdf/effect-of-egg-yolk-and-soybean-lecithin-on-tris-based-1vo9a8dhsy.pdf DOI: https://doi.org/10.9775/kvfd.2013.10248
Valcácia Silva, S., Trindade Soares, A., Batista, A. M., Costa Almeida, F., Ferreira Nunes, J., Alves Peixoto, C., & Pessoa Guerra, M. M. (2013). Vitamin E (Trolox) addition to Tris-egg yolk extender preserves ram spermatozoon structure and kinematics after cryopreservation. Animal Reproduction Science, 137(1–2), 37–44. https://doi.org/10.1016/j.anireprosci.2012.12.002 DOI: https://doi.org/10.1016/j.anireprosci.2012.12.002
Valente, S. S., Pereira, R. M., Baptista, M. C., Marques, C. C., Vasques, M. I., Silva Pereira, M. V. C., Horta, A. E. M., & Barbas, J. P. (2010). In vitro and in vivo fertility of ram semen cryopreserved in different extenders. Animal Reproduction Science, 117(1–2), 74–77. https://doi.org/10.1016/j.anireprosci.2009.04.007 DOI: https://doi.org/10.1016/j.anireprosci.2009.04.007
Vieira de Souza, C., Zandonadi Brandão, F., Dantas Rodrigues Santos, J., Angélico Pereira Alfradique, V., Moreira Barbosa dos Santos, V., Da Cruz Morais, M. C., Cerqueira Rangel, P. S., Amaral da Silva, A., & Gonçalves Souza-Fabjan, J. M. (2019). Effect of different concentrations of l-carnitine in extender for semen cryopreservation in sheep. Cryobiology, 89, 104–108. https://doi.org/10.1016/j.cryobiol.2019.05.009 DOI: https://doi.org/10.1016/j.cryobiol.2019.05.009
Watson, P. F. (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reproduction, Fertility and Development, 7(4), 871–891. https://doi.org/10.1071/RD9950871 DOI: https://doi.org/10.1071/RD9950871
Yániz, J. L., Mateos, J. A., & Santolaria, P. (2012). Tris buffer improves fluorescence yield of ram spermatozoa when evaluating membrane integrity. Microscopy Research and Technique, 75(4), 520–523. https://doi.org/10.1002/jemt.21086 DOI: https://doi.org/10.1002/jemt.21086
Zalazar, L., Iniesta-Cuerda, M., Sánchez-Ajofrín, I., Garde, J. J., Soler Valls, A. J., & Cesari, A. (2020). Recombinant SPINK3 improves ram sperm quality and in vitro fertility after cryopreservation. Theriogenology, 144, 45–55. https://doi.org/10.1016/j.theriogenology.2019.12.019 DOI: https://doi.org/10.1016/j.theriogenology.2019.12.019
Zhang, L., Wang, X., Jiang, C., Sohail, T., Sun, Y., Sun, X., Wang, J., & Li, Y. (2024). Effects of different diluents and freezing methods on cryopreservation of Hu ram semen. Veterinary Sciences, 11(6), Article 251. https://doi.org/10.3390/vetsci11060251 DOI: https://doi.org/10.3390/vetsci11060251
Zhu, Z., Zhao, H., Cui, H., Adetunji, A. O., & Min, L. (2023). Resveratrol improves the frozen-thawed ram sperm quality. Animals, 13(24), Article 3887. https://doi.org/10.3390/ani13243887 DOI: https://doi.org/10.3390/ani13243887
Additional Files
Published
Issue
Section
Categories
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
1. Proposed policy for open access journals
Authors who publish in this journal accept the following conditions:
a. Authors retain the copyright and assign to the journal the right to the first publication, with the work registered under the attribution, non-commercial and no-derivative license from Creative Commons, which allows third parties to use what has been published as long as they mention the authorship of the work and upon first publication in this journal, the work may not be used for commercial purposes and the publications may not be used to remix, transform or create another work.
b. Authors may enter into additional independent contractual arrangements for the non-exclusive distribution of the version of the article published in this journal (e.g., including it in an institutional repository or publishing it in a book) provided that they clearly indicate that the work was first published in this journal.
c. Authors are permitted and encouraged to publish their work on the Internet (e.g. on institutional or personal pages) before and during the review and publication process, as it may lead to productive exchanges and faster and wider dissemination of published work (see The Effect of Open Access).
How to Cite
