Genetic diversity analysis of Indonesian soybean genotypes based on morphological characteristics and SSR markers
DOI:
https://doi.org/10.15517/6xn1r608Keywords:
genetic background, genetic distance, genetic markers, morphological charactersAbstract
Introduction. Soybean is an important crop due to its high protein and oil content. Yellow-seeded varieties are preferred for the production of food products. Genetic diversity analysis is an important step in the selection of parental lines for breeding programs. Objective. To analyze genetic diversity of Indonesian soybean genotypes based on morphological characteristics and SSR markers. Materials and methods. Field evaluation was conducted at Bogor, Indonesia, March-June 2023. Morphological data were analyzed with principal component analysis (PCA) and principal coordinate analysis (PCoA). The genotypes were then analyzed using 14 SSR markers. Results. PCA revealed that PC1-PC4 explained 72.63 % of total genetic variation. SSR marker analysis showed excellent characteristics such as high allele number and PIC values. Genotypes grouped into two clusters with clear diversity distinction. Four genotype pairs showed high genetic diversity with favorable morphological traits related to yield and yield components. The genotype-pairs are potential as parental candidates for use in breeding programs. Conclusion. Morphological and SSR marker characterizations clearly differentiate Indonesian soybean genotypes and can identify genotype-pairs with high genetic diversity which are useful as parent candidates in breeding purposes. Both data analyses complemented each other well for better diversity findings.
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