In vitro ruminal degradation of Tithonia diversifolia

Authors

  • Gary Alex Meza-Bone Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agropecuarias, Avenida Quito km 1 ½ Vía Santo Domingo de los Tsáchilas, Quevedo, Los Ríos, Ecuador. Author https://orcid.org/0000-0002-6299-5643
  • Carlos Javier Meza-Bone Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agropecuarias, Avenida Quito km 1 ½ Vía Santo Domingo de los Tsáchilas, Quevedo, Los Ríos, Ecuador. Author https://orcid.org/0000-0002-3911-2259
  • Juan Humberto Avellaneda-Cevallos Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agropecuarias, Avenida Quito km 1 ½ Vía Santo Domingo de los Tsáchilas, Quevedo, Los Ríos, Ecuador. Author https://orcid.org/0000-0002-1805-4803
  • Luis Alberto Godoy-Montiel Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agropecuarias, Avenida Quito km 1 ½ Vía Santo Domingo de los Tsáchilas, Quevedo, Los Ríos, Ecuador. Author https://orcid.org/0000-0002-0551-9974
  • Marcos Antonio Barros-Rodríguez Universidad Técnica de Ambato, Facultad de Ciencias Agropecuarias, Sector el Tambo-La Universidad, Vía a Quero, 1801334, Cevallos, Ecuador. Author https://orcid.org/0000-0002-9608-5912
  • Fernando Jines-Fernández Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agropecuarias, Avenida Quito km 1 ½ Vía Santo Domingo de los Tsáchilas, Quevedo, Los Ríos, Ecuador. Author https://orcid.org/0000-0001-6909-1658

DOI:

https://doi.org/10.15517/am.v33i1.43206

Keywords:

forage, animal nutrition, bushy, cutting ages, cell wall

Abstract

Introduction. The Mocache canton (Ecuador) is a humid tropical zone, with a dry season of six months, which affects the availability and quality of forage, a situation that reduces the productive and reproductive capacity of ruminants. Objective. To evaluate the apparent in vitro ruminal degradation parameters of dry matter (DMD), organic matter (OMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD) in Tithonia diversifolia harvested at four different cutting ages during the dry season. Materials and methods. The research was carried out at the “La María” Experimental Farm of the Universidad Tecnica Estatal de Quevedo, Ecuador, between August and October 2017. Four rumen fistulated bulls were used. A complete randomized design (CRD) was used. The treatments were: Tithonia diversifolia at 30 days of cutting (T1), Tithonia diversifolia at 45 days of cutting (T2), Tithonia diversifolia at 60 days of cutting (T3), and Tithonia diversifolia at 75 days of cutting (T4). The incubation times were: 3, 6, 12, 24, 48, and 72 hours with four replicates per treatment. The variables evaluated were: DMD, OMD, NFDD, and AFDD. Results. The ruminal degradation parameters showed statistical differences between treatments (p<0.05), with T1 the best DMD, OMD, NDFD, and ADFD were obtained with 36.66, 35.44, 43.16, and 41.55 %, respectively). Conclusion. The highest DMD, OMD, NDFD, and ADFD parameters were associated with the cutting ages and the structural components of the bromatological composition. The cutting age at 30 days in Tithonia diversifolia influenced the potential and the effective nutrients degradation. As the cutting ages increased, the ruminal degradation parameters decreased.

Downloads

Download data is not yet available.

References

ANKOM Technology. (2010). Operator`s manual “Daisy” incubator. ANKOM Technology.

Ascencio-Rojas, L., Valles-de la Mora, B., Castillo-Gallegos, E., & Ibrahim, M. (2018). In situ ruminal degradation and effective degradation of foliage from six tree species during dry and rainy seasons in Veracruz, Mexico. Agroforestry Systems, 93, 123–133. http://doi.org/10.1007/s10457-018-0184-z

Association of Official Analytical Chemists. (2007). Official methods of analysis (18th Ed). Association of Official Analytical Chemists.

Barros-Rodríguez, M., Oña-Rodríguez, J., Mera-Andrade, E., Artieda- Rojas, J., Curay-Quispe, S., Avilés-Esquivel, & Guishca-Cunuhay, C. (2017). Degradación ruminal de dietas a base de biomasa pos-cosecha de Amaranthus cruentus: Efecto sobre los protozoos del rumen y producción de gas in vitro. Revista de Investigaciones Veterinarias del Perú, 28(4), 812–821. http://doi.org/10.15381/rivep.v28i4.13931

Barros-Rodríguez, M. A., Solorio-Sánchez, F. J., Sandoval-Castro, C. A., Klieve, A., Rojas-Herrera, R. A., Briceño-Poot, E. G., & Ku-Vera, J. C. (2015). Rumen function in vivo and in vitro in sheep fed Leucaena leucocephala. Tropical Animal Health and Production, 47(4), 757–764. https://doi.org/10.1007/s11250-015-0790-y

Delgado, D. C., La O, O., Chongo, B., Galindo, J., Obregón, Y., & Aldama, A. I. (2001). Cinética de la degradación ruminal in situ de cuatro árboles forrajeros tropicales: Leucaena leucocephala, Enterolobium cyclocarpum, Sapindus saponaria y Gliricidia sepium. Revista Cubana de Ciencia Agrícola, 35(2), 141–145.

Dong, S. -Z., Azarfar, A., Zou, Y., Li, S. -L., Wang, Y. -J., & Cao, Z. -J. (2017). Effects of sequence of nylon bags rumen incubation on kinetics of degradation in some commonly used feedstuffs in dairy rations. Journal of Integrative Agriculture, 16(1), 162–168. https://doi.org/10.1016/S2095-3119(16)61438-7

Estrada, X., Ibrahim, M., Camero, A., Abarca, S., & Hidaldo, C. (1998). Degradación ruminal de forrajes tropicales cuando se sustituye king grass Pennisetum purpureum * Pennisetum typhoides por morera Morus alba. Avances de Investigación, 5(17-18), 34–38. http://hdl.handle.net/11554/6016

González-Castillo, J. C., Hahn Von-Hessberg, C. M., & Narváez-Solarte, W. (2014). Características botánicas de Tithonia diversifolia (Asterales: Asteraceae) y su uso en la alimentación animal. Boletín Científico. Centro de Museos. Museo de Historia Natural, 18(2), 45–58. http://190.15.17.25/boletincientifico/downloads/Boletin(18)2_4.pdf

Gutiérrez, P., Rocha, L., Reyes-Sánchez, N., Paredes, V., & Mendieta-Araica, B. (2012). Ruminal degradation rate of Moringa oleifera foliage in reyna cattle using in sacco technique. Revista Científica La Calera, 12(18), 37–44. https://cenida.una.edu.ni/pperiodicas/ppl51g984.pdf

Gutiérrez, D., Borjas-Rojas, R., Rodríguez-Hernández, R., Rodríguez, Z., Stuart, R., & Sarduy, L. (2015). Evaluation of the chemical composition and in situ degradability of mixed silage with Pennisetum purpureum cv Cuba CT-169: Moringa oleifera. Revista Avances en Investigación Agropecuaria, 19(3), 7–16. http://ww.ucol.mx/revaia/portal/pdf/2015/sept/1.pdf

Instituto Nacional de Meteorología e Hidrología. (2017). Información agrometeorológica de la finca experimental “La María”. Instituto Nacional de Meteorología e Hidrología.

Keir, B., Van, B. D., Preston, T. R., & Ørskov, E. R. (1997). Nutritive value of leaves from tropical trees and shrubs. 1. In vitro gas production and in sacco rumen degradability. Livestock Research for Rural Development, 9(4), Article 35. http://www.lrrd.org/lrrd9/4/bren941.htm

La O, O., Chongo, B., Delgado, D., Ruiz, T. E., Elías, A., Stuart, J. R., & Torres, V. (2003). Degradabilidad ruminal de materia seca y nitrógeno total de seis ecotipos del género Leucaena leucocephala. Revista Cubana de Ciencia Agrícola, 37(3), 267–272. https://www.redalyc.org/pdf/1930/193018048007.pdf

La O, O., González, H., Orozco, A., Castillo, Y., Ruiz, O., Estrada, A., Gutiérrez, E., Bernal, H., Valenciaga, D., Castro, B., & Hernánez, Y. (2012) Chemical composition, in situ rumen degradability, and in vitro digestibility of Tithonia diversifolia ecotypes of interest for ruminant feeding. Cuban Journal of Agricultural Science, 46(1), 47–53.

La O-León, O., Valenciaga Gutiérrez, D., Ruiz Vázquez, T., Ruiz Barrera, O., Castillo Castillo, Y., González García, H., Rodríguez Muela, C., Hernández, D. A., Chongo García, B., Arzola Álvarez, C., & Cairo Sotolongo, J. (2008). Efecto de la edad de corte en la capacidad fermentativa in vitro y la dinámica de degradación ruminal in situ de Tithonia diversifolia Orestes. Zootecnia Tropical, 26(3), 243–247.

Mejía-Díaz, E., Mahecha-Ledesma, L., & Angulo-Arizala, J. (2016). Tithonia diversifolia: especie para ramoneo en sistemas silvopastoriles y métodos para estimar su consumo. Agronomía Mesoamericana, 28(1), 289–302. http://doi.org/10.15517/am.v28i1.22673

Medina, M. G., García, D. E., González, M. E., Cova, J. L., & Morantinos, P. (2009). Variables morfo-estructurales y de calidad de la biomasa de Tithonia diversifolia en la etapa inicial de crecimiento. Zootecnia Tropical, 27(2), 121–134. https://tspace.library.utoronto.ca/bitstream/1807/64348/1/zt09015.pdf

Menke, K. H., & Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 5–7.

Nieves, D., Terán, O., Cruz, L., Mena, M., Gutiérrez, F., & Ly, J. (2011). Nutrients digestibilty in Tithonia diversifolia foliage in fattening rabbits. Tropical and Subtropical Agroecosystems, 14(1), 309–320. http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/483

Ørskov, E.R. (2002). Trails and trails in livestock research. Abeerden.

Ørskov, E. R., & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal Agricultural Science, Cambridge, 92(2), 499–503. https://doi.org/10.1017/S0021859600063048

Peng, Q., Khan, N. A., Wang, Z., & Yu, P. (2014). Relationship of feeds protein structural makeup in common prairie feeds with protein solubility, in situ ruminal degradation and intestinal digestibility. Animal Feed Science Technology, 194, 58–70. https://doi.org/10.1016/j.anifeedsci.2014.05.004

Razz, R., Clavero, T., & Vergara-López, J. (2004 Cinética de degradación in situ de la Leucaena leucocephala y Panicum máximum. Revista Científica, FCV-LUZ, 14(5), 424–430. https://www.researchgate.net/publication/242619149_CINETICA_DE_DEGRADACION_IN_SITU_DE_LA_Leucaena_leucocephala_Y_Panicum_maximum_In_situ_Degradation_Kinetics_of_Leucaena_leucocephala_and_Panicum_maximum

Rodríguez-García, I. (2017). Potencialidades de Tithonia diversifolia (Hemsl.) Gray en la alimentación animal. Livestock Research for Rural Development, 29(4), Artículo 63. http://www.lrrd.org/lrrd29/4/idal29063.html

Salem, A. Z. M., Gado, H. M., Colombatto, D., & Elghandour, M. M. Y. (2013). Effects of exogenous enzymes on nutrient digestibility, ruminal fermentation and growth performance in beef steers. Livestock Science, 154(1-3), 69–73. https://doi.org/10.1016/j.livsci.2013.02.014

Soto, S. (2007). Digestibilidad in vitro en forrajes tropicales a diferentes edades de rebrote (Tesis de Licenciatura, no publicada). Universidad EARTH.

Soto, S., Rodríguez, J. C., & Russo, R. (2009). Digestibilidad in vitro en forrajes tropicales a diferentes edades de rebrote. Tierra Tropical, 5(1), 83–89.

Statistical Analysis System. (2011). SAS Version 9.3. Procedure guide. SAS Inc.

Tan, H. Y., Sieo, C. C., Abdullah, N., Liang, J. B., Huang, X. D., & Ho, Y. W. (2011). Effects of condensed tannins from Leucaena on methane production, rumen fermentation and populations of methanogens and protozoa in vitro. Animal Feed Science Technology, 169(3-4), 185–193. https://doi.org/10.1016/j.anifeedsci.2011.07.004

Tobía, C., Rojas, A., Villalobos, E., Soto, H., & Uribe, L. (2004). Sustitución parcial el alimento balanceado por ensilaje de soya y su efecto en la producción y calidad de la leche de vaca, en el trópico húmedo de Costa Rica. Agronomía Costarricense, 28(2), 27–35.

Torres, N., Mendoza, G. D., Bárcena, J. R., González, S. S., Loera, O., Salem, A. Z. W., & Lara, A. (2013). Effect of a fibrolytic enzymatic extract from Cellulomonas flavigena on in vitro degradation and in vivo digestibility and productive performance of lambs. Animal Nutrition and Feed Science Technology, 13, 583–592.

Valenciaga, D., López, J. R., Galindo, J., Ruíz, T., & Monteagudo, F. (2018). Cinética de degradación ruminal de materiales vegetales de Tithonia diversifolia recolectados en la región oriental de Cuba. Livestock Research for Rural Development, 30(11), Artículo 186. http://www.lrrd.org/lrrd30/11/daiky30186.html

Van-Soest, P. J., Roberton, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Published

03-11-2021

How to Cite

Meza-Bone, G. A., Meza-Bone, C. J., Avellaneda-Cevallos, J. H., Godoy-Montiel, L. A., Barros-Rodríguez, M. A., & Jines-Fernández, F. (2021). In vitro ruminal degradation of Tithonia diversifolia. Agronomía Mesoamericana, 33(1), 43206. https://doi.org/10.15517/am.v33i1.43206